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Updated: Sep 8, 2025

Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay
Published on: June 9, 2017
Proximity Ligation Assay for Detection of R-Loop Complexes upon DNA Damage
Adele Alagia1, Ruth F Ketley1, Monika Gullerova2
1Sir William Dunn School of Pathology, University of Oxford, Oxford, UK.
Abstract:
In situ Proximity Ligation Assay (PLA ) can be used to detect the close proximity (less than ~40 nm) of two biological molecules of interest in cells. Here we report the application of this method for the specific detection of R-loop interacting proteins and RNA modifications in close proximity to R-loops in non-damage and ionizing radiation (IR) induced DNA damage conditions.
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