Topology of RNA:DNA Hybrids and R-Loops in Yeast
Yathish Jagadheesh Achar1,2, Marco Foiani3,4
1IFOM (Fondazione Istituto FIRC di Oncologia Molecolare), Milan, Italy. yathish@cdfd.org.in.
Abstract:
RNA:DNA hybrids are generated naturally behind the elongating RNA polymerase as a transcriptional intermediate. However, prolonged persistence of these structures challenges the integrity of the genome by creating R-loops and by interfering with DNA replication and other chromatin related processes. Precise mapping and characterization of their distribution across the genome has been a major challenge to understand the genesis of RNA:DNA hybrids and their conversion into genotoxic intermediates. Here we provide the detailed protocol for mapping RNA:DNA hybrid across the Saccharomyces cerevisiae genome.
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