Related Experiment Video
Updated: Sep 8, 2025
06:58
Global Identification of Co-Translational Interaction Networks by Selective Ribosome Profiling
Published on: October 7, 2021
2.6K
Genome-Wide Native R-Loop Profiling by R-Loop Cleavage Under Targets and Tagmentation (R-Loop CUT&Tag)
Honghong Wang1, Conghui Li1, Kaiwei Liang2
1Department of Pathophysiology, School of Basic Medical Sciences, Wuhan University, Wuhan, China.
Methods in Molecular Biology (Clifton, N.J.)
|June 15, 2022
Summary
This study introduces R-loop CUT&Tag, a novel method for profiling DNA-RNA structures called R-loops. This technique accurately maps R-loops in various genomic regions, aiding disease research.
Area of Science:
- Molecular Biology
- Genomics
- Epigenetics
Background:
- R-loops are three-stranded nucleic acid structures (DNA-RNA hybrid and single-stranded DNA) involved in crucial cellular processes like transcription and DNA repair.
- Aberrant R-loop formation is linked to human diseases, including neurological disorders and cancer, highlighting the need for precise profiling methods.
Purpose of the Study:
- To develop and validate a convenient and sensitive method for profiling native R-loops across the genome.
- To enable comprehensive analysis of R-loop functions in both physiological and pathological contexts.
Main Methods:
- Introduced R-loop CUT&Tag, a method combining a DNA-RNA hybrid sensor (GST-His6-2×HBD or S9.6 antibody) with a pA-Tn5-based cleavage under targets and tagmentation (CUT&Tag) approach.
- The method requires as few as 0.5 million cells for sensitive detection of R-loops.
Main Results:
- R-loop CUT&Tag successfully detected native R-loops with high sensitivity.
- The method accurately mapped R-loops to specific genomic regions, including promoters, gene bodies, and enhancers.
Conclusions:
- R-loop CUT&Tag provides a powerful and convenient tool for sensitive, genome-wide profiling of native R-loops.
- This method will advance the understanding of R-loop roles in gene regulation and human diseases.
Related Concept Videos
02:24Ribosome Profiling
3.6K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.6K
03:21RNA-seq
10.4K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
10.4K