Related Experiment Video
Updated: Sep 8, 2025
08:57
Using Phylogenetic Analysis to Investigate Eukaryotic Gene Origin
Published on: August 14, 2018
16.0K
Phylogenomics of the Phylum Proteobacteria: Resolving the Complex Relationships
Vaibhav Sharma1, Amit Vashishtha1, Arsha Liz M Jos1
1Sri Venkateswara College, University of Delhi, Benito Juarez Road, Dhaula Kuan, New Delhi, Delhi, 110021, India.
Current Microbiology
|June 15, 2022
Summary
This study reevaluates Proteobacteria classification, finding concatenated conserved protein trees most accurate for bacterial phylogeny. Results suggest reclassifying Magnetococcales as Etaproteobacteria, improving bacterial classification systems.
Area of Science:
- Microbiology
- Bacterial Phylogenetics
- Taxonomy
Background:
- Proteobacteria is a large, diverse bacterial phylum with significant economic importance.
- A clear and scientifically sound classification system for Proteobacteria is urgently needed for accurate characterization.
Purpose of the Study:
- To reevaluate the current classification system of Proteobacteria.
- To suggest necessary revisions based on comprehensive phylogenetic analyses.
Main Methods:
- Construction of phylogenetic trees using 16S rRNA genes and 85 conserved protein sequences.
- Whole genome-based phylogenetic analysis using CVtree3.0.
- Amino acid identity matrix and concatenated gene trees were also employed.
Main Results:
- Confirmed polyphyly between Deltaproteobacteria (Desulfurella acetivorans) and Epsilonproteobacteria.
- Identified polyphyly within Syntrophobacterales and splitting of Thiotrichales across different trees.
- Controversial placement of Rhodocyclales, Oceonospirilalles, and Chromatiales highlighted.
Conclusions:
- Strongly support reclassification of Magnetococcales as a distinct class, Etaproteobacteria.
- Concatenated trees of conserved proteins are deemed more accurate for phylogenetic analysis.
- Further studies and revisions are needed for several controversial phylogenetic groups.
More Related Videos
08:09An Aquatic Microbial Metaproteomics Workflow: From Cells to Tryptic Peptides Suitable for Tandem Mass Spectrometry-based Analysis
Published on: September 15, 2015
8.9K
08:03Heuristic Mining of Hierarchical Genotypes and Accessory Genome Loci in Bacterial Populations
Published on: December 7, 2021
2.3K
Related Concept Videos
01:26Bacterial Phylum Proteobacteria
130
Proteobacteria, one of the largest and most diverse bacterial phyla, encompasses a wide range of Gram-negative bacteria distinguished by their outer membrane composed of lipopolysaccharides. These microorganisms exhibit various metabolic capabilities, including phototrophy, chemolithotrophy, and heterotrophy, and thrive in diverse environments from soil to aquatic systems and host-associated niches. The phylum is divided into six classes: Alphaproteobacteria, Betaproteobacteria,...
130
01:26Bacterial Phylum Verrucomicrobiota
102
The phylum Verrucomicrobiota comprises at least four characterized orders, with most species classified within the order Verrucomicrobiotales. Members of this phylum are either aerobic or facultatively aerobic, with the ability to ferment sugars. A notable exception is the genus Methylacidiphilum, which consists of aerobic methanotrophs. Additionally, some Verrucomicrobiota establish symbiotic relationships with protists. These bacteria are widely distributed across various environments,...
102
01:24Diversity of Archaea II
79
Archaea, one of the three domains of life, exhibit remarkable diversity and adaptability, thriving in both extreme and moderate environments. Historically, most identified archaea have been classified into two major phyla: Euryarchaeota and Crenarchaeota. However, recent molecular studies have expanded this classification to include three additional phyla: Thaumarchaeota, Nanoarchaeota, and Korarchaeota, each exhibiting unique characteristics and ecological roles.Thaumarchaeota: Mesophiles...
79
02:54Evolutionary Relationships through Genome Comparisons
6.1K
Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...
6.1K
01:21Three-Domain System of Life
210
Ribosomal RNA (rRNA) sequence analysis revealed three distinct groups of cells: eukaryotes, bacteria, and archaea. In 1978, Carl R. Woese proposed the concept of domains, a taxonomic level above kingdoms, to differentiate these groups. He suggested that archaea and bacteria, despite their similar appearance, represent separate domains. Domains differ in rRNA, membrane lipid structure, transfer RNA, and antibiotic sensitivity.In this classification, animals, plants, and fungi belong to the...
210
01:26Bacterial Phylum Planctomycetes
82
Planctomycetes are a group of morphologically distinct bacteria predominantly classified into two orders: Planctomycetales and Brocadiales. These gram-negative bacteria exhibit unique features, including division by budding and the presence of stalks or appendages. Their cells are often found in rosette arrangements, and they are notable for possessing an S-layer in their cell envelope, which is relatively uncommon among bacteria. Additionally, Planctomycetes frequently exhibit intracellular...
82