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A versatile embedding medium for freeform bioprinting with multi-crosslinking methods.

Qi Li1,2, Zhuoran Jiang3, Liang Ma1,2

  • 1State Key Laboratory of Fluid Power and Mechatronic Systems, Zhejiang University, Hangzhou 310058, People's Republic of China.

Biofabrication
|June 15, 2022
PubMed
Summary
This summary is machine-generated.

This study introduces a novel embedding medium for bioprinting, overcoming limitations of existing materials. The new medium supports diverse bioinks and crosslinking methods, enabling complex tissue regeneration.

Keywords:
3D bioprintingembedding mediumfreeform writingtissue regeneration

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Area of Science:

  • Biomaterials Engineering
  • Tissue Engineering
  • Bioprinting Technology

Background:

  • Conventional extrusion-based bioprinting faces challenges balancing material printability and biocompatibility.
  • Existing embedding mediums have limitations including narrow printing temperature ranges, poor bioink/crosslinker compatibility, and difficult removal.

Purpose of the Study:

  • To develop a versatile embedding medium for freeform bioprinting that addresses limitations of current materials.
  • To enable the printing of complex, heterogeneous tissue structures with high fidelity and cell viability.

Main Methods:

  • Formulation of a new embedding medium using hydrophobically modified hydroxypropylmethyl cellulose and Pluronic F-127.
  • Characterization of tunable thermoresponsive rheological properties for a programmable printing window.
  • Utilizing polyethylene glycol 400 for facile medium removal and developing an enhanced adhesion writing method.

Main Results:

  • The new medium exhibits adjustable hydrophobic/hydrophilic associations, allowing for a programmable printing window and compatibility with various bioinks.
  • Successfully printed millimetric complex tubular structures with high shape fidelity and cell viability.
  • Demonstrated patterning of five bioinks with up to five crosslinking methods into arbitrary geometries within the single medium, showcasing potential for heterogeneous tissue regeneration.

Conclusions:

  • This versatile embedding medium offers excellent compatibility with multiple crosslinking methods and a tunable printing window.
  • The developed medium opens new avenues for fabricating complex structures essential for heterogeneous tissue regeneration.