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Related Experiment Video

Updated: Sep 8, 2025

Single-Cell Resolution Three-Dimensional Imaging of Intact Organoids
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Systematically quantifying morphological features reveals constraints on organoid phenotypes.

Lauren E Beck1, Jasmine Lee1, Christopher Coté1

  • 1Department of Bioengineering, School of Engineering and Applied Sciences, University of Pennsylvania, Philadelphia, PA, USA.

Cell Systems
|June 15, 2022
PubMed
Summary
This summary is machine-generated.

Organoid morphology is shaped by tissue-specific constraints, not just culture conditions. This study develops a framework to identify these constraints, revealing how cell number scales with cyst size in kidney organoids.

Keywords:
design principlesmorphologyorganoids

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Area of Science:

  • * Developmental Biology
  • * Organoid Science
  • * Quantitative Biology

Background:

  • * Organoids mimic complex 3D organ structures, offering insights into self-organization principles.
  • * Organoid morphology often reflects the native organ, suggesting inherent tissue-specific constraints.
  • * Understanding these constraints is crucial for accurate organoid modeling and engineering.

Purpose of the Study:

  • * To establish a quantitative framework for identifying morphological constraints in organoids.
  • * To investigate how tissue-specific constraints govern organoid morphology under varying conditions.
  • * To apply this framework to kidney and intestinal organoids.

Main Methods:

  • * Developing a computational framework to quantify organoid morphological features from microscopy images.
  • * Applying perturbation experiments to organoids cultured under various conditions.
  • * Analyzing scaling relationships and parameter limits within organoid populations.

Main Results:

  • * Identified specific constraints on Madin-Darby canine kidney cyst morphology, including scaling relationships.
  • * Demonstrated that cell number increases with cyst size, while cell size remains constant.
  • * Showed that these constraints are dynamic, varying with cyst age and culture conditions.
  • * Observed similar constraint patterns in intestinal organoids.

Conclusions:

  • * Organoid morphology is governed by identifiable, tissue-specific constraints beyond culture conditions.
  • * The developed quantitative framework enables systematic identification and analysis of these constraints.
  • * Findings provide a foundation for precise organoid engineering and understanding developmental processes.