The Effect of Cigarette Smoke through Biofilters with Natural Plant Materials on Mice MDA Level

Background: Cigarette smoke causes various health problems. Therefore, it is necessary to overcome the problem. One solution to these problems is biofilters' use to capture free radicals from cigarette smoke. This study aims to discover the effect of cigarette smoke exposure through a biofilter made from dates, olives, and pomegranates on mice's MDA levels. Methods: The experimental study was performed to compare the result of five different treatments. Exposure of 150 ml of cigarette smoke was given every day using a suction tool (15 times each mouse) for 28 days. It was performed with five controls treatment: negative, positive, date biofilter, pomegranate biofilter, and olive biofilter. Furthermore, the data were analyzed using the one-way ANOVA followed by the Duncan test to discover differences in mice's MDA levels from each treatment. Results: The measurement of MDA levels is using the TBA (Thiobarbitaric Acid) test. The sample is obtained from 1.8 grams of the mices' liver in each treatment.The results showed an effect of using date, pomegranate, and olive biofilters on mice's MDA levels. It shows that the best MDA levels were found in date biofilter treatment with a value of 224 ng/ml with a p-value of 0.023. Conclusion: The results showed that the MDA levels with the Dates and Olives biofilter treatment were better than the negative control. Therefore, these treatment makes the harmful content of cigarette smoke can be minimized.