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Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy
Published on: October 4, 2019
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Bioinformatics analysis of potential glioblastoma circular RNA sponge network
Liwen Zhao1,2, Pengfei Zhang3, Yang Nan1,4
1Department of Neurosurgery, Tianjin Medical University General Hospital Airport Site, Tianjin, China.
Translational Cancer Research
|June 16, 2022
Summary
Circular RNAs (circRNAs) are emerging molecules in glioblastoma research. This study establishes a circRNA-microRNA regulatory network, identifying potential therapeutic targets like hsa_circ_0001073, hsa-miR-1248, and RNF11 for glioblastoma treatment.
Area of Science:
- Oncology
- Molecular Biology
- Bioinformatics
Background:
- Circular RNA (circRNA) is recognized as a significant functional molecule in glioblastoma.
- The precise functions and regulatory mechanisms of circRNAs in glioblastoma remain largely unelucidated.
- This study aims to explore the regulatory roles of circRNAs in glioblastoma through bioinformatics analysis.
Purpose of the Study:
- To establish a molecular sponge regulatory network for circular RNAs in glioblastoma.
- To identify potential therapeutic targets for glioblastoma based on circRNA and microRNA expression patterns.
- To elucidate the functional enrichment of target genes within the identified regulatory network.
Main Methods:
- Utilized Gene Expression Omnibus datasets for circRNA extraction and analysis.
- Employed CircInteractome and miRabel databases for predicting microRNA binding and gene targets.
- Integrated Chinese Glioma Gene Atlas and The Cancer Genome Atlas databases for expression and survival trend analysis.
- Performed Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses for functional annotation.
- Constructed and visualized the regulatory network using R and Cytoscape software.
Main Results:
- Identified three differentially expressed circRNAs (hsa_circ_0000219, hsa_circ_0001073, hsa_circ_0070700) and two microRNAs (hsa-miR-1248, hsa-miR-1290) associated with glioblastoma prognosis.
- Screened potential microRNA targets including ARHGEF7, CELA2b, RNF11, YPEL1, and ZNF37a.
- Functional enrichment analysis revealed significant involvement in signal transduction, cell plasma membrane, ATP binding, and calcium signaling pathways.
Conclusions:
- A circRNA-molecular sponge regulatory network involving hsa-miR-1248 and hsa-miR-1290 was successfully established.
- Specific circRNAs (hsa_circ_0001073, hsa_circ_0070700, hsa_circ_0000219), microRNAs (hsa-miR-1248, hsa-miR-1290), and a target gene (RNF11) show potential as glioblastoma therapeutic targets.
- Further experimental validation is required to confirm the function and significance of these identified molecules in glioblastoma.
