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Related Experiment Video

Updated: Sep 8, 2025

Epicardial Outgrowth Culture Assay and Ex Vivo Assessment of Epicardial-derived Cell Migration
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Dynamic Epicardial Contribution to Cardiac Interstitial c-Kit and Sca1 Cellular Fractions.

C Pogontke1,2, J A Guadix1,2, A M Sánchez-Tévar1,2

  • 1Department of Animal Biology, Faculty of Sciences, University of Málaga, Málaga, Spain.

Frontiers in Cell and Developmental Biology
|June 16, 2022
PubMed
Summary
This summary is machine-generated.

Cardiac interstitial cells (CICs) expressing c-Kit and Sca1 markers have unknown origins. This study traces their lineage, revealing Wt1-epicardial cells contribute minimally to c-Kit+ CICs but significantly to Sca1+ CICs, indicating heterogeneity.

Keywords:
Sca1+ cellsblood-borne cellsc-Kit+ (CD117+) cellscardiac interstitiumepicardium

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Related Experiment Videos

Last Updated: Sep 8, 2025

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Area of Science:

  • Cardiovascular Biology
  • Developmental Biology
  • Cell Biology

Background:

  • The cardiac interstitium contains diverse cell types, including those expressing stem cell markers like c-Kit and Sca1.
  • The developmental dynamics, embryonic origin, and heterogeneity of these c-Kit-positive and Sca1-positive cardiac interstitial cells (CICs) remain largely uncharacterized.
  • Previous assumptions about these cells being true cardiac stem cells have been revised.

Purpose of the Study:

  • To investigate the embryonic origin and lineage relationship of c-Kit-positive and Sca1-positive CIC subpopulations.
  • To test the hypothesis that both c-Kit-positive and Sca1-positive CIC subpopulations are derived from the Wilms' tumor 1 (Wt1) epicardial lineage.

Main Methods:

  • Utilized genetic cell lineage tracing techniques to track cell origins.
  • Employed immunohistochemistry and Fluorescence-Activated Cell Sorting (FACS) for cell characterization.

Main Results:

  • Approximately 50% of cardiac c-Kit-positive cells at embryonic day 15.5 originated from the Wt1-lineage, decreasing significantly by postnatal day 7.
  • A substantial portion of c-Kit-positive CICs exhibited markers indicative of blood-borne cells.
  • The proportion of Sca1-positive cells within the Wt1-lineage increased postnatally, with Sca1 expression found in 90% of adult epicardial-derived endothelial cells and 60% of cardiac fibroblasts.

Conclusions:

  • The Wt1-epicardial lineage contributes minimally to c-Kit-positive CICs throughout cardiac development and adulthood.
  • A significant fraction of adult epicardial-derived cells are Sca1-positive, highlighting the heterogeneity of CICs based on their embryonic origin.
  • Understanding this heterogeneity is crucial for developing diagnostic and prognostic tools for cardiac health and disease.