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Comparison of Molecular Characteristics Between Methicillin-Resistant and -Susceptible Staphylococcus aureus Clinical

Hongying Zhu1, Hong Luo1, Qiaoshi Zhong1

  • 1Department of Clinical Laboratory Medicine, Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, People's Republic of China.

Infection and Drug Resistance
|June 16, 2022
PubMed
Abstract

Insights

Methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-susceptible Staphylococcus aureus (MSSA) infections are a global concern. MSSA isolates carry diverse virulence factors, necessitating intensified efforts to combat these infections.

Area of Science:

  • Microbiology
  • Epidemiology
  • Genomics

Background:

  • Methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-susceptible Staphylococcus aureus (MSSA) pose significant global public health challenges.
  • Understanding the molecular evolution and dissemination of S. aureus is crucial for effective control strategies.

Purpose of the Study:

  • To compare the molecular characteristics of MSSA and MRSA isolates.
  • To identify differences in antimicrobial resistance determinants and virulence gene profiles between MSSA and MRSA.

Main Methods:

  • Whole-genome sequencing (WGS) was employed to analyze 74 MSSA and 102 MRSA clinical isolates collected between 2016 and 2020.
  • Molecular epidemiology, antimicrobial resistance, and virulence gene profiles were assessed.

Main Results:

  • Twenty distinct sequence types (STs) were identified in MRSA, with ST59, ST630, and ST338 being most common. MSSA predominantly belonged to ST188 and ST7.
  • Specific toxin genes (clfA, sek, seq) were associated with MRSA, while others (splA/B, clfB, map, sdrC/D, sem-sen-seo-seu) were more frequent in MSSA.
  • The tst gene was common in CC1 and CC72, and lukE/D was prevalent in CC7, CC15, and CC88, but absent in CC59.

Conclusions:

  • MSSA isolates possess a wide array of virulence factors, indicating a need for intensified efforts against MSSA infections.
  • Comparative epidemiological studies are essential to understand the co-evolution and interaction of MSSA and MRSA populations.
Keywords:
methicillin-resistant Staphylococcus aureusmethicillin-susceptible Staphylococcus aureusmolecular characteristicsresistancevirulencewhole-genome sequencing

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