Negative regulation of miR-1288-3p/KRT4 axis through a circular RNA in oral cancer

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Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
11:44

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

7.7K

Lu Wang1, Xin Ma1, Jie Yu1

  • 1Department of stomatology, Henan Provincial People's Hospital, Zhengzhou University People's Hospital, Henan University People's Hospital, Zhengzhou, Henan, China.

Journal of Biochemical and Molecular Toxicology
|June 16, 2022
PubMed

Insights

Circular RNA hsa_circ_0096042 inhibits oral squamous cell carcinoma (OSCC) progression by targeting the miR-1288-3p/KRT4 axis. Overexpression of hsa_circ_0096042 suppressed tumor growth and promoted apoptosis in OSCC.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Circular RNAs (circRNAs) are implicated in oral squamous cell carcinoma (OSCC) pathogenesis.
  • The specific role of hsa_circ_0096042 in OSCC remains largely unelucidated.

Purpose of the Study:

  • To investigate the function and mechanism of hsa_circ_0096042 in oral squamous cell carcinoma.
  • To explore the regulatory relationship between hsa_circ_0096042, miR-1288-3p, and KRT4 in OSCC.

Main Methods:

  • Quantitative reverse-transcription polymerase chain reaction (qRT-PCR) for gene expression analysis.
  • Cell viability, proliferation, and apoptosis assays (CCK-8, colony formation, western blot).
  • In vivo xenograft experiments, bioinformatics prediction, luciferase reporter assays, RIP, and RNA pull-down assays to confirm molecular interactions.

Main Results:

  • Hsa_circ_0096042 and KRT4 were downregulated, while miR-1288-3p was upregulated in OSCC tissues and cell lines.
  • Overexpression of hsa_circ_0096042 inhibited OSCC cell proliferation, viability, and in vivo tumor growth, while promoting apoptosis.
  • Hsa_circ_0096042 directly targets miR-1288-3p, and KRT4 is a target gene of miR-1288-3p, forming the miR-1288-3p/KRT4 axis.

Conclusions:

  • Hsa_circ_0096042 functions as an antitumor circRNA in oral squamous cell carcinoma.
  • The hsa_circ_0096042/miR-1288-3p/KRT4 axis plays a critical role in regulating OSCC progression.
  • Upregulation of miR-1288-3p promotes OSCC and counteracts the antitumor effects of hsa_circ_0096042.
Keywords:
KRT4OSCChsa_circ_0096042miR-1288-3poral cancer

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