Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Fractures: Bone Repair01:27

Fractures: Bone Repair

3.6K
Treatment for a fracture is based on the type of break, the bone affected, and the patient's age.
Minor fractures with no bone displacement are treated by immobilizing the fractured bone using a cast or splint. However, in the case of fractures with displaced bones, the broken bones are repositioned before immobilization to ensure successful healing without deformation and loss of function. The realignment of fractured bone ends is performed through a process called reduction. If the...
3.6K
Tissue Renewal without Stem Cells01:23

Tissue Renewal without Stem Cells

1.8K
After cellular or tissue damage, the resident stem cells present in the human body can locally repair and regenerate the damaged tissue or organ. However, even though some tissues do not have stem cells, they can repair and regenerate with the help of pre-existing cells. For example, beta cells of the pancreas and hepatocytes of the liver can divide to renew and regenerate the tissue. Here, both cell division and cell death are well regulated by homeostasis.
However, failure of such a system...
1.8K
Phases of Wound Repair01:28

Phases of Wound Repair

6.3K
Following injury, the integrity of the injured tissues must be reestablished. For example, in skin tissue, wound repair involves coordination among resident skin cells, blood mononuclear cells, extracellular matrix, growth factors, and cytokines to complete the healing cascade.
Formation of Blood Clot
In case of deep injuries, trauma to blood vessels results in blood loss. In the meantime, phospholipids released from the ruptured endothelial cellular membrane are converted into arachidonic...
6.3K
Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

2.7K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
2.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Standard operating procedures for quality control of oral biospecimens at the Korea Oral Biobank Network.

Journal of periodontal & implant science·2025
Same author

Development of MDM2-Targeting PROTAC for Advancing Bone Regeneration.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2025
Same author

Standard operating procedures for quality control of oral biospecimens at the Korea Oral Biobank Network.

Journal of periodontal & implant science·2025
Same author

Cervical enamel projections from a periodontal perspective: A scoping review.

Clinical anatomy (New York, N.Y.)·2024
Same author

Standard operating procedures for the collection, processing, and storage of oral biospecimens at the Korea Oral Biobank Network.

Journal of periodontal & implant science·2023
Same author

Influence of irradiation distance on the mechanical performances of resin composites polymerized with high-irradiance light curing units.

Biomaterials research·2022

Related Experiment Video

Updated: Sep 7, 2025

Peptides from Phage Display Library Modulate Gene Expression in Mesenchymal Cells and Potentiate Osteogenesis in Unicortical Bone Defects
07:53

Peptides from Phage Display Library Modulate Gene Expression in Mesenchymal Cells and Potentiate Osteogenesis in Unicortical Bone Defects

Published on: December 10, 2010

18.1K

Vitronectin-Derived Peptide Promotes Reparative Dentin Formation.

C Park1,2, M Song3, S Y Kim2

  • 1Department of Oral Biochemistry and Program in Cancer and Developmental Biology, Dental Research Institute, School of Dentistry, Seoul National University, Seoul, Korea.

Journal of Dental Research
|June 16, 2022
PubMed
Summary
This summary is machine-generated.

A novel peptide, VnP-16, effectively promotes reparative dentin formation in direct pulp-capping procedures. This biocompatible agent enhances odontoblast differentiation and mineralization without causing inflammation, suggesting its potential as a superior dental material.

Keywords:
dental pulpdental pulp exposurepeptidespulp cappingregenerationvitronectin

More Related Videos

Development of a Direct Pulp-capping Model for the Evaluation of Pulpal Wound Healing and Reparative Dentin Formation in Mice
07:07

Development of a Direct Pulp-capping Model for the Evaluation of Pulpal Wound Healing and Reparative Dentin Formation in Mice

Published on: January 12, 2017

11.4K
Accessing the Cytotoxicity and Cell Response to Biomaterials
09:46

Accessing the Cytotoxicity and Cell Response to Biomaterials

Published on: July 8, 2021

3.8K

Related Experiment Videos

Last Updated: Sep 7, 2025

Peptides from Phage Display Library Modulate Gene Expression in Mesenchymal Cells and Potentiate Osteogenesis in Unicortical Bone Defects
07:53

Peptides from Phage Display Library Modulate Gene Expression in Mesenchymal Cells and Potentiate Osteogenesis in Unicortical Bone Defects

Published on: December 10, 2010

18.1K
Development of a Direct Pulp-capping Model for the Evaluation of Pulpal Wound Healing and Reparative Dentin Formation in Mice
07:07

Development of a Direct Pulp-capping Model for the Evaluation of Pulpal Wound Healing and Reparative Dentin Formation in Mice

Published on: January 12, 2017

11.4K
Accessing the Cytotoxicity and Cell Response to Biomaterials
09:46

Accessing the Cytotoxicity and Cell Response to Biomaterials

Published on: July 8, 2021

3.8K

Area of Science:

  • Biomaterials Science
  • Dental Regenerative Medicine
  • Cell Biology

Background:

  • Exposed dental pulp requires vital pulp capping with biocompatible materials for healing and reparative dentin formation.
  • A previously studied vitronectin-derived peptide (VnP-16) promotes osteoblast differentiation and inhibits osteoclast activity.
  • The potential of VnP-16 in promoting dentinogenesis and pulp healing requires further investigation.

Purpose of the Study:

  • To evaluate the efficacy of VnP-16 as a direct pulp-capping agent.
  • To assess VnP-16's effects on odontoblast differentiation, mineralization, and reparative dentin formation in a rat model.
  • To compare VnP-16 with established pulp-capping agents like Mineral Trioxide Aggregate (MTA) and recombinant human bone morphogenetic protein 2 (rhBMP-2).

Main Methods:

  • In vitro assessment of VnP-16 cytotoxicity and effects on human dental pulp cells (hDPSCs) regarding differentiation and mineralization.
  • In vivo pulp exposure model in rat teeth using VnP-16, MTA, and rhBMP-2.
  • Histological analysis at 2 and 4 weeks to evaluate inflammatory responses and reparative dentin formation.

Main Results:

  • VnP-16 demonstrated no cytotoxicity and enhanced odontoblast-like cell differentiation and mineralization in hDPSCs, comparable to vitronectin.
  • In the rat model, VnP-16 induced mild or no inflammation at 2 and 4 weeks.
  • While MTA showed early reparative dentin, VnP-16 induced similar amounts of thicker, homogeneous reparative dentin compared to MTA and rhBMP-2 at 4 weeks, without inflammation.

Conclusions:

  • VnP-16 promotes odontoblast differentiation and mineralization, leading to high-quality reparative dentin formation.
  • VnP-16 exhibits a favorable safety profile with minimal inflammatory response in vivo.
  • VnP-16 presents a promising alternative direct pulp-capping agent for enhanced dental tissue regeneration.