Related Experiment Video

Updated: Sep 7, 2025

Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes
08:32

Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes

Published on: May 23, 2025

451

Exploring the genetic space of the DNA damage response for cancer therapy through CRISPR-based screens

Jordan Wilson1,2, Joanna I Loizou1,2

  • 1Center for Cancer Research, Comprehensive Cancer Centre, Medical University of Vienna, Austria.

Molecular Oncology
|June 16, 2022
PubMed

Insights

Synthetic lethality and viability reveal cancer vulnerabilities and resistances in DNA damage response. CRISPR screens and chemical compounds systematically identify and target these interactions for cancer treatment.

Area of Science:

  • Genetics and Genomics
  • Cancer Biology
  • Drug Discovery

Background:

  • Synthetic lethality and viability are key concepts for understanding cancer's DNA damage response.
  • Genetic interactions have been studied in model organisms but are newly prioritized for cancer therapeutics.
  • Technological advances in genetic screening have enabled the study of complex gene interactions.

Purpose of the Study:

  • To discuss synthetic lethal and viable interactions within the DNA damage response.
  • To present how CRISPR-based genetic screens and chemical compounds can identify and target these interactions.
  • To highlight the therapeutic potential of targeting genetic interactions in cancer.

Main Methods:

  • Review of synthetic lethality and viability concepts in cancer.
  • Discussion of CRISPR-based genetic screening methodologies.
  • Analysis of chemical compound applications in targeting DNA damage response pathways.

Main Results:

  • Synthetic lethal and viable interactions offer insights into cancer vulnerabilities.
  • CRISPR screens enable systematic identification of gene interactions.
  • Chemical compounds can be used to target identified synthetic lethal interactions.

Conclusions:

  • Synthetic lethality and viability are promising therapeutic strategies for cancer.
  • CRISPR technology and chemical compounds are powerful tools for targeting cancer vulnerabilities.
  • Further research into DNA damage response interactions can lead to novel cancer treatments.
Keywords:
CRISPR-Cas9 screensDNA damage responsecancer therapydrug discoverysynthetic lethalitysynthetic viability

More Related Videos

Pooled CRISPR-Based Genetic Screens in Mammalian Cells09:05

Pooled CRISPR-Based Genetic Screens in Mammalian Cells

Published on: September 4, 2019

22.2K
Performing an In Vitro Genome-Wide CRISPR Knockout Screen in Chimeric Antigen Receptor T Cells07:37

Performing an In Vitro Genome-Wide CRISPR Knockout Screen in Chimeric Antigen Receptor T Cells

Published on: January 31, 2025

1.0K

Related Concept Videos

Genetic Screens02:46

Genetic Screens

Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing  genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which...
5.1K
CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
52.8K
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
51.4K
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
207