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Evaluation of microwave-thawed canine plasma for transfusion
Journal of the American Veterinary Medical Association
|April 1, 1987
Summary
Microwave thawing of fresh-frozen canine plasma is a safe and effective method. This rapid thawing technique preserves key coagulation factors and does not cause adverse physiological effects in dogs, making it a viable alternative to water bath thawing for veterinary plasma.
Area of Science:
- Veterinary Medicine
- Hematology
- Biotechnology
Background:
- Fresh-frozen plasma (FFP) is crucial in veterinary critical care.
- Traditional thawing methods, like water baths, are time-consuming.
- Rapid thawing methods are needed to improve FFP accessibility.
Purpose of the Study:
- To evaluate the efficacy of microwave thawing for canine plasma.
- To assess the impact of microwave thawing on key coagulation factors.
- To determine the safety and physiological effects of infusing microwave-thawed canine plasma.
Main Methods:
- Canine plasma units were thawed using a microwave oven (15.4 x 10-second intervals).
- Coagulation parameters (aPTT, PT, fibrinogen, FVIII, vWF) were compared to water bath-thawed plasma.
- Five dogs received autologous microwave-thawed plasma, with physiological parameters monitored for 24 hours.
Main Results:
- Microwave thawing did not significantly alter activated partial thromboplastin times, one-stage prothrombin times, fibrinogen, factor-VIII coagulant activity, or von Willebrand factor antigen.
- No significant changes in vital signs (temperature, heart rate, respiration) were observed post-infusion.
- Hematological parameters (PCV, total protein, platelets, RBC, WBC counts) remained stable after plasma infusion.
Conclusions:
- Microwave thawing is a rapid and effective method for preparing canine plasma.
- This technique preserves the functional integrity of essential coagulation factors.
- Infusion of microwave-thawed plasma is safe and well-tolerated in dogs, with no adverse physiological effects.