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Updated: Sep 7, 2025
Two-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification
Published on: March 31, 2021
Digital Droplet PCR to Track SARS-CoV-2 Outbreak in a Hospital Transitional Care Unit
Kathryn Linton1, Lisa K Oberding2, Linda Ward1
1Infection Prevention and Control, Alberta Health Services, Calgary, Alberta Canada.
Abstract:
We describe an outbreak of SARS-CoV-2 on a transition unit composed of elderly patients awaiting placement. Environmental and patient sample analyses using digital droplet PCR (ddPCR) suggested possible fomite transmission and a high viral burden source from a few individual patients. This outbreak illustrates challenges inherent to this specific patient population.
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