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Related Experiment Videos

Evaluation of microscopic examination for bacteriuria.

H D Yu

    Zhonghua Minguo Wei Sheng Wu Xue Za Zhi = Chinese Journal of Microbiology
    |March 1, 1978
    PubMed
    Summary

    Microscopic examination of urine sediment for pus cells and bacteria is a reliable, inexpensive method for detecting significant bacteriuria. Combining both cell counts and visible organisms improves diagnostic accuracy in routine urinalysis.

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    Area of Science:

    • Clinical Microbiology
    • Urology
    • Diagnostic Pathology

    Background:

    • Routine urine examination is crucial for diagnosing urinary tract infections.
    • Accurate and cost-effective methods for detecting significant bacteriuria are needed.

    Purpose of the Study:

    • To evaluate a simple, reliable, and inexpensive method for demonstrating significant bacteriuria in routine urine analysis.
    • To compare the efficacy of microscopic examination versus bacteriological cultures.

    Main Methods:

    • 206 urine samples were analyzed using parallel microscopic observation and bacteriological cultures.
    • Microscopic examination focused on pus cells and bacteria in centrifuged urine deposits.
    • Criteria included >5 pus cells/high power field and visible organisms in methylene blue stain.

    Main Results:

    • Microscopic examination of both pus cells and bacteria was more satisfactory than examining either element alone for significant bacteriuria.
    • The combined microscopic criteria achieved a sensitivity of 79%.
    • A false positive rate of 13% was observed with the proposed microscopic method.

    Conclusions:

    • Microscopic examination of urine sediment for pus cells and bacteria offers a practical approach for detecting significant bacteriuria.
    • This method presents a viable, inexpensive alternative for routine urinalysis, aiding in timely diagnosis and treatment.

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