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Mass spectrometry-based draft of the mouse proteome
Piero Giansanti1, Patroklos Samaras1, Yangyang Bian1,2
1Chair of Proteomics and Bioanalytics, Technical University of Munich, Freising, Germany.
Nature Methods
|June 16, 2022
Summary
Researchers created a comprehensive mouse proteome and phosphoproteome map from 41 tissues. This resource aids in understanding gene expression, species differences, and identifying drug/radiation response markers in pancreatic cancer.
Area of Science:
- Biomedical Research
- Proteomics
- Genomics
Background:
- The laboratory mouse is a crucial model in biomedical research.
- Molecular reference maps are essential for understanding complex biological systems.
- A comprehensive proteome and phosphoproteome map of the mouse is needed.
Purpose of the Study:
- To present a quantitative draft of the mouse proteome and phosphoproteome.
- To provide insights into gene expression, isoforms, and phosphorylation sites.
- To identify molecular markers for drug and radiation response in pancreatic ductal adenocarcinoma (PDAC).
Main Methods:
- Construction of a quantitative mouse proteome and phosphoproteome from 41 healthy tissues.
- Proteogenomic comparison across mouse, human, and Arabidopsis.
- Integration of phenotypic drug and radiation response data with PDAC cell line proteomes.
Main Results:
- Tissue- and cell-type resolved profiles provide evidence for 17,000 genes, thousands of isoforms, and 50,000 phosphorylation sites.
- Comparative analysis revealed conserved and divergent gene expression regulation mechanisms.
- Identification of molecular markers associated with sensitivity and resistance in PDAC cell lines.
Conclusions:
- The generated mouse proteome and phosphoproteome atlas is a valuable resource for biomedical research.
- The data offers insights into species-specific functions and gene expression regulation.
- This atlas can facilitate the discovery of novel therapeutic targets for PDAC.
