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Updated: Sep 7, 2025

Isolating Intestinal Stem Cells from Adult Drosophila Midguts by FACS to Study Stem Cell Behavior During Aging
Published on: December 16, 2014
Intestinal stem cell aging signature reveals a reprogramming strategy to enhance regenerative potential
Christian M Nefzger1,2,3,4, Thierry Jardé1,3,5, Akanksha Srivastava6
1Department of Anatomy and Developmental Biology, Monash University, Clayton, VIC, Australia.
Abstract:
The impact of aging on intestinal stem cells (ISCs) has not been fully elucidated. In this study, we identified widespread epigenetic and transcriptional alterations in old ISCs. Using a reprogramming algorithm, we identified a set of key transcription factors (Egr1, Irf1, FosB) that drives molecular and functional differences between old and young states. Overall, by dissecting the molecular signature of aged ISCs, our study identified transcription factors that enhance the regenerative capacity of ISCs.
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