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Related Experiment Video

Updated: Sep 7, 2025

Automated and High-throughput Microbial Monoclonal Cultivation and Picking Using the Single-cell Microliter-droplet Culture Omics System

Automated and High-throughput Microbial Monoclonal Cultivation and Picking Using the Single-cell Microliter-droplet Culture Omics System

Published on: March 14, 2025

A Wipe-Based Stool Collection and Preservation Kit for Microbiome Community Profiling.

Hui Hua¹, Cem Meydan¹, Evan E Afshin¹

¹Thorne HealthTech, New York, NY, United States.

Frontiers in Immunology

|June 17, 2022

Summary

A novel wipe-based method simplifies fecal sample collection for microbiome analysis. This approach, using DESS solution, offers stable room-temperature storage and transport, comparable to traditional methods.

Keywords:

DESS metagenomics microbiome stool collection wipe

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Last Updated: Sep 7, 2025

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Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing

Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing

Published on: March 19, 2018

Area of Science:

Microbiome Research
Molecular Biology
Genomics

Background:

Traditional stool collection methods are often complex and require specialized handling.
Standardized and user-friendly fecal sample collection is crucial for accurate microbiome analysis.

Purpose of the Study:

To introduce and validate a novel, wipe-based method for fecal sample collection and stabilization.
To assess the efficacy of this new method for metagenomics analysis compared to existing techniques.

Main Methods:

Collected 72 fecal samples using four preservation methods: freezing, room temperature storage, a commercial kit, and a novel DESS (dimethyl sulfoxide, ethylenediaminetetraacetic acid, sodium chloride) wipe.
Performed metagenomic sequencing and analyzed taxonomic abundance, bacterial metabolic pathways, and diversity metrics.

Main Results:

The DESS wipe method demonstrated high correlation (R²=0.96) with frozen samples for species abundance across all kingdoms.
Shannon diversity (3.1-3.3) and species richness (151-159) were maintained, comparable to frozen samples.
DESS wipe performance was similar to a commercial preservation kit (R²=0.98), with samples clustering by subject across all methods.

Conclusions:

The DESS wipe method is a validated, stable approach for room-temperature fecal specimen collection and transport.
This novel method simplifies sample handling for microbiome studies, enhancing accessibility and potentially improving data consistency.