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Cell-cycle-regulated translation of histone mRNA in Physarum plasmodia

T G Laffler, J Carrino

    Journal of Bacteriology
    |May 1, 1987
    PubMed

    Insights

    Histone mRNA accumulates before S phase in Physarum polycephalum. However, histone synthesis is restricted to S phase, indicating delayed translation of stored mRNA.

    Area of Science:

    • Cell Biology
    • Molecular Biology
    • Biochemistry

    Background:

    • Histone proteins are crucial for DNA packaging and replication.
    • Histone mRNA accumulation precedes DNA synthesis in many organisms.
    • The regulation of histone synthesis during the cell cycle is not fully understood.

    Purpose of the Study:

    • To investigate the temporal regulation of histone mRNA translation during the cell cycle in Physarum polycephalum.
    • To determine when stored histone mRNA becomes available for protein synthesis.

    Main Methods:

    • Cell cycle synchronization of Physarum polycephalum.
    • Quantitative analysis of histone mRNA levels.
    • In vitro translation assays using cytoplasmic extracts.

    Main Results:

    • Histone mRNA accumulation was observed 3 hours prior to S phase.
    • Histone synthesis was strictly limited to the S phase.
    • Cytoplasmic histone mRNA from G2 phase was translatable in vitro.

    Conclusions:

    • Histone mRNA is stored in the cytoplasm during G2 phase.
    • Translation of stored histone mRNA is initiated at the onset of S phase.
    • This regulation ensures that histones are synthesized concurrently with DNA replication.

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