CultureLED: A 3D printer-based LED illumination cultivation system for multi-well culture plates

Microalgae are a source of high value products such as pigments, lipids and carbohydrates. Microalgae cultivation techniques have evolved and improved, but a vast amount of research is still needed to achieve a better understanding of these microorganisms. Due to this, there is a growing need for affordable, flexible, and easy to control research systems and protocols. 3D printing revolutionized design and manufacturing as it became widely available to the mass market, allowing the creation of novel forms, enabling mass customization, and supporting low-volume, distributed production. The emergence of open-source designs combined with 3D printing applications have the potential to replace and outperform standard designs and methods. This protocol describes CultureLED, a well plated cell culture system that can be mounted on an orbital shaker or positioned on a shelf. It is an open hardware design, based on low-cost commercial off-the-shelf components. It was designed for optimized production cost, simplicity, low power consumption, design flexibility, and controllable light conditions. The CultureLED utilizes light-emitting diodes and it can be used for cultivation of small sized organisms or microorganisms with different light requirements and as such, has a wide range of applications.