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Updated: Sep 7, 2025
08:56
A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues
Published on: December 5, 2016
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Transcriptome-Wide m6A Methylome and m6A-Modified Gene Analysis in Asthma
Deyang Sun1, Xiaolu Cai1, Fenglin Shen1
1The First Clinical College, Zhejiang Chinese Medical University, Hangzhou, China.
Frontiers in Cell and Developmental Biology
|June 17, 2022
Summary
This study identifies key N6-methyladenosine (m6A) modified genes involved in asthma, revealing potential new therapeutic targets. Understanding these RNA methylation changes offers novel insights for asthma treatment strategies.
Area of Science:
- Molecular Biology
- Genetics
- Immunology
Background:
- N6-methyladenosine (m6A) is a prevalent RNA modification regulating gene expression.
- Previous research suggests m6A regulators are involved in asthma, but specific modified genes remain unclear.
Purpose of the Study:
- To systematically evaluate the asthma methylome and identify m6A-modified genes.
- To uncover key RNA methylation-related genes in asthma pathogenesis.
Main Methods:
- Utilized methylated RNA immunoprecipitation sequencing (MeRIP-seq) and RNA sequencing (RNA-seq).
- Performed differential analysis of methylation peaks and gene expression.
- Validated findings using data from the U-BIOPRED program (87 controls, 411 asthma cases).
Main Results:
- Identified significant differences in m6A methylation peaks (416 upregulated, 152 downregulated) and gene expression (2,505 upregulated, 4,715 downregulated) in asthma.
- Screened 14 differentially expressed genes related to RNA methylation modification.
- Validated three key m6A-modified genes (BCL11A, MATK, CD300A) predominantly in exons and enriched in 3' UTR.
Conclusions:
- m6A modification plays a significant role in asthma.
- BCL11A, MATK, and CD300A are identified as key m6A-modified genes in asthma.
- Targeting these m6A-modified genes may offer novel therapeutic strategies for asthma.
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