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Non-competitive fluorescence polarization immunosensing for CD9 detection using a peptide as a tracer
Kazuki Takahashi1, Shunsuke Chida1, Thanawat Suwatthanarak2
1Graduate School of Chemical Sciences and Engineering, Hokkaido University, Kita 13 Nishi 8, Kita-ku, Sapporo 060-8628, Japan.
Lab on a Chip
|June 17, 2022
Summary
Researchers developed a new non-competitive fluorescence polarization immunoassay (NC-FPIA) using a peptide tracer. This rapid method can detect CD9 and CD9-expressing exosomes, showing promise for point-of-need diagnostics.
Area of Science:
- Biochemistry
- Analytical Chemistry
- Biotechnology
Background:
- Immunoassays are crucial for quantifying biomolecules, but often require complex procedures.
- Exosomes, particularly markers like CD9, are significant in disease diagnostics.
- Developing rapid, simple assays for point-of-need applications remains a challenge.
Purpose of the Study:
- To report the first non-competitive fluorescence polarization immunoassay (NC-FPIA) utilizing a peptide tracer.
- To demonstrate the application of NC-FPIA for the detection of CD9 and CD9-expressing exosomes.
- To highlight the potential of this assay for point-of-need diagnostic applications.
Main Methods:
- Development of a novel non-competitive fluorescence polarization immunoassay (NC-FPIA).
- Utilized a synthesized peptide as a fluorescent tracer for the immunoassay.
- Applied the NC-FPIA to detect the exosome marker CD9 and exosomes derived from HeLa cells.
Main Results:
- Successfully developed and validated an NC-FPIA using a peptide tracer.
- Quantified CD9 protein using the developed NC-FPIA.
- Detected exosomes expressing CD9 derived from HeLa cells with the NC-FPIA.
Conclusions:
- The developed NC-FPIA offers a simple, rapid, and sensitive method for target quantification.
- This peptide-based NC-FPIA successfully detected CD9 and CD9-expressing exosomes.
- The assay platform holds significant potential for diverse point-of-need applications, including clinical diagnostics and food safety.
