Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cancer Stem Cells and Tumor Maintenance02:40

Cancer Stem Cells and Tumor Maintenance

5.0K
Early diagnosis and treatment can often cure cancer. However, even with treatment, residual cells called cancer stem cells (CSC) might remain, often causing tumor recurrence. These cancer stem cells possess the potential for self-renewal and multi-lineage differentiation and are often responsible for the therapeutic resistance displayed in most cancers.
Cancer stem cells are thought to originate from tissue-specific normal stem cells or progenitor cells. The normal stem cells usually reside in...
5.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Droplet-based single-cell pairing for high-throughput interaction mapping of antigen-receptor combinations.

Science advances·2025
Same author

Correction to "Optochemical Control of mTOR Signaling and mTOR-Dependent Autophagy".

ACS pharmacology & translational science·2025
Same author

Quantifying Protein-Nucleic Acid Interactions for Engineering Useful CRISPR-Cas9 Genome-Editing Variants.

Methods in molecular biology (Clifton, N.J.)·2024
Same author

Author Correction: High-throughput screening of genetic and cellular drivers of syncytium formation induced by the spike protein of SARS-CoV-2.

Nature biomedical engineering·2024
Same author

High-throughput screening of genetic and cellular drivers of syncytium formation induced by the spike protein of SARS-CoV-2.

Nature biomedical engineering·2023
Same author

Facilitating Machine Learning-Guided Protein Engineering with Smart Library Design and Massively Parallel Assays.

Advanced genetics (Hoboken, N.J.)·2023

Related Experiment Video

Updated: Sep 7, 2025

Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes
08:32

Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes

Published on: May 23, 2025

451

CRISPR screening in cancer stem cells.

Bei Wang1,2, Alan S L Wong1,2,3

  • 1Laboratory of Combinatorial Genetics and Synthetic Biology, School of Biomedical Sciences, The University of Hong Kong, Hong Kong SAR, China.

Essays in Biochemistry
|June 17, 2022
PubMed
Summary

Cancer stem cells (CSCs) drive tumor growth and relapse. Advanced CRISPR screening methods coupled with CSC models offer new ways to uncover their vulnerabilities and improve cancer treatments.

Keywords:
CRISPRCancer stem cellshigh-throughput screening

More Related Videos

Evaluation of Abnormal Growth-related Genes of Hematopoietic Stem and Progenitor Cells by Combining CRISPR/Cas9 Technology with Cell Counting
07:01

Evaluation of Abnormal Growth-related Genes of Hematopoietic Stem and Progenitor Cells by Combining CRISPR/Cas9 Technology with Cell Counting

Published on: May 2, 2025

309
In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity
07:52

In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity

Published on: November 2, 2020

6.6K

Related Experiment Videos

Last Updated: Sep 7, 2025

Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes
08:32

Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes

Published on: May 23, 2025

451
Evaluation of Abnormal Growth-related Genes of Hematopoietic Stem and Progenitor Cells by Combining CRISPR/Cas9 Technology with Cell Counting
07:01

Evaluation of Abnormal Growth-related Genes of Hematopoietic Stem and Progenitor Cells by Combining CRISPR/Cas9 Technology with Cell Counting

Published on: May 2, 2025

309
In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity
07:52

In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity

Published on: November 2, 2020

6.6K

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Cancer stem cells (CSCs) are crucial for tumor development, metastasis, and treatment resistance.
  • Understanding CSC self-renewal and survival mechanisms is key to improving cancer therapy outcomes.
  • Current knowledge of CSC vulnerabilities remains limited, hindering effective therapeutic strategies.

Purpose of the Study:

  • To review the application of CRISPR/Cas9 screening in identifying genetic factors in cancer.
  • To explore the potential of advanced CRISPR screening systems combined with CSC models.
  • To highlight emerging opportunities for discovering CSC-specific vulnerabilities.

Main Methods:

  • Utilizing clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 screening technology.
  • Employing cancer stem cell (CSC) models for experimental studies.
  • Analyzing genetic determinants that regulate CSC self-renewal and survival.

Main Results:

  • CRISPR screening is a powerful tool for identifying genes involved in cancer progression.
  • Coupling CRISPR screening with CSC models facilitates the discovery of novel CSC vulnerabilities.
  • This approach holds promise for uncovering previously understudied aspects of CSC biology.

Conclusions:

  • Advanced CRISPR screening systems are vital for dissecting CSC biology.
  • Identifying CSC vulnerabilities through these methods can lead to more effective cancer treatments.
  • Further research coupling CRISPR screening and CSC models is essential for therapeutic advancements.