Elevational shifts in foliar-soil δ¹⁵ N in the Hengduan Mountains and different potential mechanisms

The natural abundance of stable nitrogen isotopes (δ¹⁵ N) provides insights into the N dynamics of terrestrial ecosystems, the determination of which is considered an effective approach for gaining a better understanding ecosystem N cycling. However, there is currently little information available regarding the patterns and mechanisms underlying the variation in foliar-soil δ¹⁵ N among mountain ecosystems. In this study, we examined the determinants of foliar-soil δ¹⁵ N in association with N transportation rates along an elevational gradient in the Hengduan Mountains. Despite the relatively high levels of available N produced from high N fixation and mineralization, we detected the lowest levels of foliar δ¹⁵ N at 3500 m a.s.l., reflecting the stronger vegetation N limitation at medium high elevations. The enhanced vegetation N limitation was driven by the combined effects of higher microbial immobilization and inherent plant dynamic (the shifts of δ¹⁵ N in vegetation preference, including vegetation community) with changing climate along the elevational gradient. Unexpectedly, we established that soil δ¹⁵ N was characterized by an undulating rise and uncoupled correlation with foliar δ¹⁵ N with increasing elevation, thereby indicating that litter input might not be a prominent driver of soil δ¹⁵ N. Conversely, soil nitrification and denitrification were found to make a more pronounced contribution to the pattern of soil δ¹⁵ N along the elevational gradient. Collectively, our results serve to highlight the importance of microbial immobilization in soil N dynamics and provide novel insights that will contribute to enhancing our understanding of N cycling as indicated by foliar-soil δ¹⁵ N along elevational gradients.