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Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria
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Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria

Published on: May 8, 2013

Effect of source of Mueller-Hinton agar on detection of oxacillin resistance in Staphylococcus aureus using a

J A Hindler, N L Warner

    Journal of Clinical Microbiology
    |April 1, 1987
    PubMed

    Insights

    Oxacillin screening plates reliably detect oxacillin-resistant Staphylococcus aureus. However, the specific Mueller-Hinton agar manufacturer can impact the accuracy of these crucial diagnostic tests.

    Area of Science:

    • Microbiology
    • Clinical Diagnostics
    • Antimicrobial Resistance

    Background:

    • Staphylococcus aureus is a significant pathogen, and oxacillin resistance is a growing concern.
    • Accurate and rapid detection of oxacillin resistance is crucial for effective treatment strategies.

    Purpose of the Study:

    • To evaluate the reliability of oxacillin screening agar plates for detecting oxacillin-resistant Staphylococcus aureus (ORSA).
    • To assess the influence of different Mueller-Hinton agar manufacturers on screening plate performance.

    Main Methods:

    • Dehydrated Mueller-Hinton agar from five manufacturers was used to prepare screening plates with 4% NaCl and 6 µg/mL oxacillin.
    • 137 Staphylococcus aureus isolates (109 resistant, 28 susceptible) from diverse geographic areas were tested.
    • Results were compared against minimum inhibitory concentrations (MICs) determined by broth microdilution.

    Main Results:

    • Screening plates from three manufacturers showed 100% correlation with MICs for detecting ORSA.
    • Plates from the remaining two manufacturers demonstrated 90% and 99% correlation, respectively.
    • No oxacillin-susceptible isolates grew on any screening plates, indicating high specificity.

    Conclusions:

    • Oxacillin screening agar plates are a reliable method for detecting oxacillin-resistant Staphylococcus aureus.
    • The performance of these screening plates can be influenced by the brand of dehydrated Mueller-Hinton agar used.

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