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Updated: Sep 7, 2025
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Identification of Coding and Non-coding RNA Classes Expressed in Swine Whole Blood
Published on: November 28, 2018
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Expression patterns and functional analysis of porcine lnc-34015
Shu Wang1, Haizhen Wang1, Juan Liu1
1Department of Animal Sciences, Shanxi Agricultural University, Taigu, China.
Animal Biotechnology
|June 17, 2022
Summary
This study reveals long noncoding RNA (lncRNA) lnc-34015
Area of Science:
- Molecular Biology
- Genomics
- Animal Science
Background:
- Long noncoding RNAs (lncRNAs) are crucial regulators of immune responses in animals.
- The specific functions and regulatory mechanisms of lnc-34015 in muscle development remain largely uncharacterized.
Purpose of the Study:
- To elucidate the molecular mechanisms underlying the role of lnc-34015 in porcine muscle development and immune regulation.
- To analyze the sequence characteristics, expression patterns, and regulatory network of lnc-34015.
Main Methods:
- Sequence analysis of lnc-34015.
- Prediction of lncRNA competitive endogenous RNA (ceRNA) network.
- Quantitative polymerase chain reaction (qPCR) for expression analysis.
- Bioinformatic analyses including Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment.
Main Results:
- lnc-34015 overlaps with CWF19L1 introns and has CWF19L1, PKD2L1, and CHUK as cis-regulatory genes.
- lnc-34015 interacts with 15 microRNAs (miRNAs), including miR-3646, miR-377-3p, and miR-190b-3p.
- lnc-34015 is involved in cell proliferation, stress response, transcriptional regulation, and alternative splicing, with expression patterns correlating with target genes and opposing miRNAs in muscle.
- Higher expression of lnc-34015 was observed in the porcine small intestine and IPEC-J2 cells.
Conclusions:
- lnc-34015 acts as a ceRNA to regulate the expression of CHUK, ZBTB20, and XIAP.
- These regulatory actions are implicated in controlling porcine inflammatory responses.
- lnc-34015 plays a significant role in both muscle development and immune modulation in pigs.
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