Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cancers Originate from Somatic Mutations in a Single Cell02:21

Cancers Originate from Somatic Mutations in a Single Cell

12.6K
Cancer arises from mutations in the critical genes that allow healthy cells to escape cell cycle regulation and acquire the ability to proliferate indefinitely. Though originating from a single mutation event in one of the originator cells, cancer progresses when the mutant cell lines continue to gain more and more mutations, and finally, become malignant. For example, chronic myelogenous leukemia (CML) develops initially as a non-lethal increase in white blood cells, which progressively...
12.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Immunohistochemical Loss of MTAP as a Diagnostic and Prognostic Surrogate of <i>CDKN2A/B</i> Homozygous Deletion: A Narrative Review.

Diagnostics (Basel, Switzerland)·2026
Same author

Integrative Bioinformatic Characterization of the HDAC6-Driven Cytoskeleton-Wnt Signaling Interface in Hepatocellular Carcinoma: Implications for Immune Modulation and Therapeutic Targeting.

International journal of molecular sciences·2026
Same author

Risk stratification and relapse pattern in triple-negative breast cancer with pathological complete response after neoadjuvant treatment: the European GAMBIT real-world study.

Nature communications·2026
Same author

Diagnostic Usefulness of LC-OCT in Mammary and Extramammary Paget Disease and Correlation With Vertical and Horizontal Histopathology: A Case Series.

The American Journal of dermatopathology·2026
Same author

WWOX-Related Epileptic Encephalopathy (WOREE Syndrome): Clinical Case Study and Literature Review.

Current issues in molecular biology·2026
Same author

Bioactive-Supplemented Infant Formulas and Early Gut-Immune-Endocrine Development: A Narrative Review.

International journal of molecular sciences·2026

Related Experiment Video

Updated: Sep 7, 2025

Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors
11:15

Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors

Published on: September 20, 2016

24.5K

Multiple primary malignances managed with surgical excision: a case report with next generation sequencing analysis.

Chiara Romano1,2, Sandra Di Gregorio3,4, Maria Stella Pennisi3,4

  • 1Department of Clinical and Experimental Medicine, University of Catania, 95123, Catania, Italy. chiararomano83@gmail.com.

Molecular Biology Reports
|June 17, 2022
PubMed
Summary

Next-generation sequencing (NGS) helped diagnose metachronous multiple primary malignancies (MPM) by revealing distinct molecular profiles in two distinct cancers. Despite identifying genetic variants, NGS could not correlate them with the patients favorable prognosis.

Keywords:
Colon adenocarcinomaGastric adenocarcinomaMultiple primary malignanciesMutation profilingNext generation sequencingPrediction tools

More Related Videos

Comparative Lesions Analysis Through a Targeted Sequencing Approach
08:16

Comparative Lesions Analysis Through a Targeted Sequencing Approach

Published on: November 5, 2019

6.8K
Author Spotlight: Advancements in Molecular Biomarker Testing for Non-Squamous Non-Small Cell Lung Cancer
07:59

Author Spotlight: Advancements in Molecular Biomarker Testing for Non-Squamous Non-Small Cell Lung Cancer

Published on: September 8, 2023

1.2K

Related Experiment Videos

Last Updated: Sep 7, 2025

Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors
11:15

Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors

Published on: September 20, 2016

24.5K
Comparative Lesions Analysis Through a Targeted Sequencing Approach
08:16

Comparative Lesions Analysis Through a Targeted Sequencing Approach

Published on: November 5, 2019

6.8K
Author Spotlight: Advancements in Molecular Biomarker Testing for Non-Squamous Non-Small Cell Lung Cancer
07:59

Author Spotlight: Advancements in Molecular Biomarker Testing for Non-Squamous Non-Small Cell Lung Cancer

Published on: September 8, 2023

1.2K

Area of Science:

  • Oncology
  • Genomics
  • Molecular Diagnostics

Background:

  • Multiple primary malignancies (MPM) involve distinct tumors in one individual.
  • Distinguishing MPM from metastatic disease requires advanced diagnostic tools.
  • Next-generation sequencing (NGS) offers sensitive DNA analysis for genetic discrimination.

Observation:

  • A patient presented with gastric adenocarcinoma and later developed colorectal cancer.
  • The second cancer involved multiple organs, initially suggesting metastasis.
  • Adjuvant chemotherapy proved effective for both advanced-stage diseases.

Findings:

  • NGS mutation profiling identified two distinct malignancies, confirming metachronous MPM.
  • Somatic mutations were found only in the gastric cancer.
  • Germline variants in PDGFRA, APC, and TP53 were identified but not correlated with prognosis.

Implications:

  • NGS is crucial for accurate diagnosis of complex MPM cases.
  • Molecular profiling aids in differentiating independent primary tumors.
  • Further research is needed to understand genetic drivers of unexpected treatment responses in MPM.