Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Fatty acids in breast milk during lactation in <i>Ateles geoffroyi</i> offspring in managed care and semi-free-ranging.

Current zoology·2026
Same author

The Combination of <i>Lactobacillus reuteri</i> RC-14<sup>®</sup> and <i>Lactobacillus rhamnosus</i> GR-1<sup>®</sup> Induces Anxiolytic-like and Antidepressant-like Effects via Estrogenic Receptors in Ovariectomized Rats.

Nutrients·2026
Same author

The role of flower consumption in Howler Monkey Females' diet: adjustment across reproductive states.

PeerJ·2026
Same author

Guava (Psidium guajava L.) leaf extract improves the prognosis of nonalcoholic fatty liver and biochemical parameters in rats with metabolic syndrome.

Journal of complementary & integrative medicine·2025
Same author

Chrysin enhances serotonergic and noradrenergic neurotransmission associated with antidepressant effects: A pharmacological study.

Acta pharmaceutica (Zagreb, Croatia)·2025
Same author

Estrogenic Effect of Probiotics on Anxiety and Depression: A Narrative Review.

International journal of molecular sciences·2025

Related Experiment Video

Updated: Sep 7, 2025

Automated Quantification of Hematopoietic Cell &#8211; Stromal Cell Interactions in Histological Images of Undecalcified Bone
09:31

Automated Quantification of Hematopoietic Cell – Stromal Cell Interactions in Histological Images of Undecalcified Bone

Published on: April 8, 2015

11.7K

Optimizing rat and human blood cells sampling for in silico morphometric analysis.

Isaac Zamora-Bello1, Daniel Hernandez-Baltazar2, Juan Francisco Rodríguez-Landa3

  • 1Facultad de Química Farmacéutica Biológica, Universidad Veracruzana, Xalapa, Veracruz, Mexico.

Acta Histochemica
|June 18, 2022
PubMed
Summary

Preserving blood smears with a histological resin enhances cell integrity for morphometric analysis. This method aids in detailed white blood cell descriptions and establishes reference intervals, improving diagnostic accuracy.

Keywords:
Automatic thresholdingBlood smear examinationHematic cytometryHumanSegmentation algorithmWistar rat

More Related Videos

Characterizing Mutational Load and Clonal Composition of Human Blood
07:58

Characterizing Mutational Load and Clonal Composition of Human Blood

Published on: July 11, 2019

7.5K
A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
09:34

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

6.8K

Related Experiment Videos

Last Updated: Sep 7, 2025

Automated Quantification of Hematopoietic Cell &#8211; Stromal Cell Interactions in Histological Images of Undecalcified Bone
09:31

Automated Quantification of Hematopoietic Cell – Stromal Cell Interactions in Histological Images of Undecalcified Bone

Published on: April 8, 2015

11.7K
Characterizing Mutational Load and Clonal Composition of Human Blood
07:58

Characterizing Mutational Load and Clonal Composition of Human Blood

Published on: July 11, 2019

7.5K
A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
09:34

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

6.8K

Area of Science:

  • Hematology
  • Biomedical Imaging
  • Histology

Background:

  • Morphometric Parameters of the Blood Cells (MPBC) are crucial for diagnosing mental and metabolic diseases.
  • While manual and computational methods exist for diagnosis, pre-analytical sample handling is under-evaluated.
  • Reliable clinical diagnosis depends on robust sample processing and data analysis.

Purpose of the Study:

  • To improve blood smear preservation using a histological resin.
  • To provide detailed morphometric descriptions of white blood cells.
  • To establish reference intervals for white blood cells in male Wistar rats.

Main Methods:

  • Blood smears from male Wistar rats and adult men were collected and preserved with a toluene-based synthetic resin.
  • In silico image analysis evaluated the integrity of Wright-stained cells.
  • Reference intervals were established using cell counting and segmentation algorithms.

Main Results:

  • The histological resin significantly improved the conservation of blood cell populations.
  • Morphologic features of white blood cells, including nucleus/cytoplasmic shape and granule presence, were clearly identified.
  • The resin facilitated in silico MPBC measurements by enhancing cell preservation.

Conclusions:

  • Histological resin offers a practical approach for preserving blood smears.
  • This method supports detailed morphometric analysis and establishes reference intervals.
  • The resin-based technique can improve efficiency in sample handling for MPBC measurements in various species.