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ATP synthase subunit e is a shrimp growth-associated breeding marker
Yucheng Lu1, Qingyun Liu2, Zhiqi Huang1
1Department of Biology, College of Science, Shantou University, Shantou 515063, China.
Genomics
|June 18, 2022
Summary
Researchers identified ATP synthase subunit e as a key genetic marker for accelerating growth in Penaeus vannamei shrimp. This discovery enhances shrimp breeding efficiency for faster-growing aquaculture strains.
Area of Science:
- Aquaculture genetics
- Animal breeding
- Molecular biology
Background:
- Penaeus vannamei is a globally significant aquaculture species, prized for rapid growth and flavor.
- Developing selectively bred strains requires efficient identification of growth-associated genetic markers.
- Improving breeding efficiency is crucial for meeting global seafood demand.
Purpose of the Study:
- To screen for key regulatory genes associated with fast growth in Penaeus vannamei.
- To identify reliable genetic markers for enhancing shrimp breeding programs.
- To validate candidate genes for their correlation with growth rate.
Main Methods:
- Utilized Fluorescence-Activated Cell Sorting (FACS) with 2-NBDG to measure glucose uptake and screen candidate genes.
- Performed in vitro validation of six candidate genes in 293T cells.
- Verified gene correlation with shrimp growth in a hybrid lineage and across various lineages.
Main Results:
- Screened six candidate genes using a high-throughput FACS-based method measuring glucose uptake.
- Identified ATP synthase subunit e and inhibitor of apoptosis protein expression correlating with shrimp growth speed.
- Confirmed ATP synthase subunit e as a viable shrimp growth-associated breeding marker.
Conclusions:
- ATP synthase subunit e is a promising genetic marker for accelerating Penaeus vannamei growth.
- This marker can significantly improve breeding efficiency in shrimp aquaculture.
- The study provides a foundation for marker-assisted selection in Penaeus vannamei breeding programs.
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