Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

6.9K
Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
6.9K
Peptide Bonds02:43

Peptide Bonds

76.4K
A peptide bond covalently attaches amino acids through a dehydration reaction. One amino acid's carboxyl group and another amino acid's amino group combine, releasing a water molecule. The resulting bond is the peptide bond. The products that such linkages form are peptides. As more amino acids join this growing chain, the resulting chain is a polypeptide. Each polypeptide has a free amino group at one end. This end has the N-terminal, or the amino-terminal, and the other end has a free...
76.4K
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

6.1K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
6.1K
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

6.7K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
6.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Precision medicine with car cells in acute myeloid leukemia: where are we?

Frontiers in immunology·2025
Same author

Joint, multifaceted genomic analysis enables diagnosis of diverse, ultra-rare monogenic presentations.

Nature communications·2025
Same author

Joint genotypic and phenotypic outcome modeling improves base editing variant effect quantification.

Nature genetics·2024
Same author

Epigenetic profiling reveals key genes and cis-regulatory networks specific to human parathyroids.

Nature communications·2024
Same author

Joint, multifaceted genomic analysis enables diagnosis of diverse, ultra-rare monogenic presentations.

bioRxiv : the preprint server for biology·2024
Same author

Ribosome impairment regulates intestinal stem cell identity via ZAKÉ‘ activation.

Nature communications·2022
Same journal

Demonstration of a quantum C-NOT gate in a time-multiplexed fully reconfigurable photonic processor.

Nature communications·2026
Same journal

Nonlinear quantum light source with van der Waals ferroelectric NbOX<sub>2</sub> (X = Br, I).

Nature communications·2026
Same journal

Antagonistic histone H2A variants and autonomous heterochromatin formation shape epigenomic patterns in Arabidopsis.

Nature communications·2026
Same journal

The long tail of nitrate pollution in groundwater challenges governance of global water quality.

Nature communications·2026
Same journal

Select microbial metabolites promote tau aggregation in a murine tauopathy model.

Nature communications·2026
Same journal

Warming climate has lengthened global intense tropical cyclone seasons.

Nature communications·2026
See all related articles

Related Experiment Video

Updated: Sep 7, 2025

CAPRRESI: Chimera Assembly by Plasmid Recovery and Restriction Enzyme Site Insertion
07:37

CAPRRESI: Chimera Assembly by Plasmid Recovery and Restriction Enzyme Site Insertion

Published on: June 25, 2017

11.7K

Peptide fusion improves prime editing efficiency.

Minja Velimirovic1,2, Larissa C Zanetti1,3, Max W Shen4

  • 1Division of Genetics, Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, 02115, USA.

Nature Communications
|June 18, 2022
PubMed
Summary
This summary is machine-generated.

Prime editing, a genome editing technology, can be enhanced by fusing specific peptides. These prime-enhancing peptides boost editing efficiency by improving translation, offering a valuable tool for genetic research.

More Related Videos

Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9
14:48

Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9

Published on: August 25, 2018

26.8K
OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy
08:34

OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy

Published on: February 5, 2020

6.8K

Related Experiment Videos

Last Updated: Sep 7, 2025

CAPRRESI: Chimera Assembly by Plasmid Recovery and Restriction Enzyme Site Insertion
07:37

CAPRRESI: Chimera Assembly by Plasmid Recovery and Restriction Enzyme Site Insertion

Published on: June 25, 2017

11.7K
Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9
14:48

Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9

Published on: August 25, 2018

26.8K
OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy
08:34

OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy

Published on: February 5, 2020

6.8K

Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • Prime editing offers precise genome editing but faces limitations due to low efficiency.
  • Developing strategies to enhance prime editing efficiency is crucial for its broader application.

Purpose of the Study:

  • To systematically evaluate the impact of peptide fusion on prime editing efficiency.
  • To identify specific peptides that can enhance prime editing outcomes.

Main Methods:

  • Development of a high-throughput assay, Peptide Self-Editing sequencing assay (PepSEq).
  • Screening of 12,000 peptide fusions to assess their influence on prime editing efficiency.
  • Validation of top-performing peptides in multiple cell lines and across various target sites.

Main Results:

  • Peptide fusion was demonstrated to enhance prime editing efficiency.
  • Combinations of prime-enhancing peptides showed productive synergy.
  • A dual peptide-prime editor significantly increased prime editing efficiency across diverse cellular contexts and genomic locations.

Conclusions:

  • Prime-enhancing peptides can significantly improve prime editing efficiency.
  • These peptides function by increasing translation efficiency.
  • The identified peptides represent broadly applicable tools for advancing prime editing technology.