Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA Stability01:53

RNA Stability

33.9K
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
33.9K
Nucleic Acid Structure01:25

Nucleic Acid Structure

6.9K
The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA...
6.9K
Ribosome Profiling02:24

Ribosome Profiling

3.6K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.6K
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

13.4K
Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
13.4K
Riboswitches01:56

Riboswitches

8.5K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
8.5K
Ribozymes02:47

Ribozymes

12.4K
The term ribozyme is used for RNA that can act as an enzyme. Ribozymes are mainly found in selected viruses, bacteria, plant organelles, and lower eukaryotes. Ribozymes were first discovered in 1982 when Tom Cech’s laboratory observed Group I introns acting as enzymes. This was shortly followed by the discovery of another ribozyme, Ribonulcease P, by Sid Altman’s laboratory. Both Cech and Altman received the Nobel Prize in chemistry in 1989 for their work on ribozymes.
Ribozymes can...
12.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Author Correction: Targeting a key disulfide linkage to regulate RIG-I condensation and cytosolic RNA-sensing.

Nature cell biology·2026
Same author

HetDualCL: Dual-encoder contrastive learning for heterogeneous graphs.

Neural networks : the official journal of the International Neural Network Society·2026
Same author

A Study on the efficiency of protein extraction from oxytetracycline residues using the method of electrochemical oxidation.

Environmental technology·2026
Same author

AND-Logic-Gated Aptamer Switch for Precise Targeting and Regulation of RNA G-Quadruplexes.

Angewandte Chemie (International ed. in English)·2026
Same author

The RNA-Binding Protein PARN Remodeled 3' UTR Structure Defines Poly(A)-Loading Sites to Mediate Immunoglobulin Homeostasis.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

OSK-mediated partial reprogramming induces cardiomyocyte dedifferentiation, overcomes cytokinesis barriers, and promotes post-MI endogenous cardiac regeneration.

Journal of molecular and cellular cardiology·2026
Same journal

Targeting SYK to alleviate MDSC-driven immunosuppression and augment anti-PD1 efficacy.

Science China. Life sciences·2026
Same journal

Microbial community structure and function and their linkages with methane production in sediments of thermokarst lakes on the Tibetan Plateau.

Science China. Life sciences·2026
Same journal

Roles of progestogens in influenza A virus-induced inflammatory responses.

Science China. Life sciences·2026
Same journal

RNA regulation in plants.

Science China. Life sciences·2026
Same journal

Translating genetic testing into fertility care.

Science China. Life sciences·2026
Same journal

A newly identified palmitoylation-related molecular signature acts as predictor and possible therapeutic target of atherosclerosis.

Science China. Life sciences·2026
See all related articles

Related Experiment Video

Updated: Sep 7, 2025

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
10:34

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

Published on: December 9, 2022

4.4K

Recent advances in RNA structurome.

Bingbing Xu1, Yanda Zhu1, Changchang Cao2

  • 1MOE Laboratory of Biosystems Homeostasis & Protection, Innovation Center for Cell Signaling Network, College of Life Sciences, Zhejiang University, Hangzhou, 310058, China.

Science China. Life Sciences
|June 18, 2022
PubMed
Summary
This summary is machine-generated.

Novel technologies reveal genome-wide RNA structures and functions. This review covers methods for studying the RNA structurome, including applications in SARS-CoV-2, and discusses future prospects for high-resolution studies.

Keywords:
3D structureRNA secondary structureRNA structuromeSARS-CoV-2decodingfunctiongenome-widehigh-throughput techniques

More Related Videos

Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen
11:32

Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen

Published on: May 24, 2017

12.2K
Using In Vitro and In-cell SHAPE to Investigate Small Molecule Induced Pre-mRNA Structural Changes
11:58

Using In Vitro and In-cell SHAPE to Investigate Small Molecule Induced Pre-mRNA Structural Changes

Published on: January 30, 2019

8.4K

Related Experiment Videos

Last Updated: Sep 7, 2025

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
10:34

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

Published on: December 9, 2022

4.4K
Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen
11:32

Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen

Published on: May 24, 2017

12.2K
Using In Vitro and In-cell SHAPE to Investigate Small Molecule Induced Pre-mRNA Structural Changes
11:58

Using In Vitro and In-cell SHAPE to Investigate Small Molecule Induced Pre-mRNA Structural Changes

Published on: January 30, 2019

8.4K

Area of Science:

  • Molecular Biology
  • Genomics
  • Bioinformatics

Background:

  • RNA structures are crucial for biological functions and regulation.
  • Recent advancements enable genome-wide RNA structure analysis across species.
  • Understanding RNA structure is key to deciphering biological processes.

Purpose of the Study:

  • To review key strategies for probing the RNA structurome.
  • To discuss the advantages and disadvantages of novel RNA structure-probing technologies.
  • To summarize RNA structure functionalities in various biological contexts.

Main Methods:

  • Review of existing literature on RNA structure probing technologies.
  • Analysis of applications of these technologies in diverse species, including SARS-CoV-2.
  • Synthesis of findings on RNA structure roles in biological regulation.

Main Results:

  • Overview of diverse RNA structure-probing technologies and their comparative analysis.
  • Demonstration of the utility of these technologies in dissecting viral RNA genomes (e.g., SARS-CoV-2).
  • Summary of RNA structure functions across different regulatory layers and organisms.

Conclusions:

  • Novel technologies are advancing the field of RNA structurome research.
  • RNA structures play vital roles in gene regulation, from processing to translation.
  • Future research will focus on high-resolution, single-molecule/cell studies for novel applications.