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Transcriptome Analysis of Pennisetum glaucum (L.) R. Br. Provides Insight Into Heat Stress Responses
Albert Maibam1,2, Showkat Ahmad Lone2, Sunil Ningombam1,2
1PG School, Indian Council of Agricultural Research-Indian Agricultural Research Institute, New Delhi, India.
Frontiers in Genetics
|June 20, 2022
Summary
Pearl millet (Pennisetum glaucum) genotypes show varied responses to heat stress. Genotype 841-B maintains homeostasis better than PPMI-69 under terminal heat stress, offering insights for developing resilient crops.
Area of Science:
- Plant Science
- Genomics
- Molecular Biology
Background:
- Pearl millet (Pennisetum glaucum) is a vital crop in arid regions, frequently facing heat stress.
- While tolerant during vegetative stages, terminal growth stages (flowering to grain filling) are more vulnerable to heat stress.
- Specific genotypes, like 841-B, exhibit enhanced tolerance to terminal heat stress.
Purpose of the Study:
- To investigate the molecular mechanisms underlying heat stress tolerance in contrasting pearl millet genotypes.
- To identify differentially expressed genes (DEGs) in response to heat stress at the flowering stage.
- To provide genetic resources for developing heat-resilient pearl millet varieties.
Main Methods:
- RNA sequencing was performed on two contrasting pearl millet genotypes (841-B and PPMI-69) exposed to heat stress (42°C for 6 hours).
- High-quality sequencing reads were assembled into unigenes, followed by functional annotation using Blastx, Gene Ontology, COG, and KEGG pathway analysis.
- Simple sequence repeats (SSRs) and single nucleotide polymorphisms (SNPs) were identified, and differentially expressed genes were validated using qRT-PCR.
Main Results:
- Over 274 million high-quality reads were generated, assembled into 47,310 unigenes.
- Functional analysis annotated 35,628 unigenes, with significant assignments to Gene Ontology, COG, and KEGG pathways.
- A total of 12,976 SSRs and 305,759 SNPs were identified. 2,301 DEGs were detected, with 10 selected for qRT-PCR validation.
- Genotype 841-B demonstrated a superior ability to maintain homeostasis under heat stress compared to PPMI-69.
Conclusions:
- Pearl millet genotype 841-B exhibits enhanced heat stress tolerance at the terminal growth stage.
- The identified SSRs and SNPs are valuable resources for developing genetic markers for heat tolerance.
- The study highlights key heat-responsive genes that can be utilized for engineering transgenic, heat-resilient pearl millet varieties.
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