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Updated: Sep 7, 2025
High-Throughput Measurement and Classification of Organic P in Environmental Samples
Published on: June 8, 2011
A combined approach to evaluate total phosphorus/inorganic phosphate levels in plants
Bin Ma1, Yu Liu2, Xiaoyuan Li3
1National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology & Ecology, Chinese Academy of Sciences, Shanghai, China.
Abstract:
Inorganic phosphate (Pi) and phosphorus (P) homeostasis are essential for plant growth and yield, and reliable detection of dynamic Pi/P in different tissues is important for studying their biological functions. Here, we report a combined protocol for rapid determination of Pi/P levels. We first perform 31P NMR assay to reveal the intracellular Pi distribution and then dissect the level of Pi/P by the chromogenic reaction and ICP-MS analysis. Finally, we take μXRF element fluorescence assay to achieve the visual P distribution. For complete details on the use and execution of this protocol, please refer to Ma et al. (2021).
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