Alternatives to pristane priming for ascitic fluid and monoclonal antibody production

A variety of agents were compared to pristane for priming mice prior to ascitic fluid production. Incomplete Freund's adjuvant was found to be as good as or better than pristane for priming before hybridoma cell inoculation. Complete adjuvant was also useful while priming with proteose-peptone; thioglycollate, corn oil or mineral oil elicited only minimum amounts of ascitic fluid after hybridoma injection.