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A Structural Atlas of the Developing Zebrafish Telencephalon Based on Spatially-Restricted Transgene Expression.

Katherine J Turner1, Thomas A Hawkins1, Pedro M Henriques2

  • 1Department of Cell and Developmental Biology, University College London, London, United Kingdom.

Frontiers in Neuroanatomy
|June 20, 2022
PubMed
Summary

Zebrafish telencephalon development involves eversion, a process impacting cell positioning. This study maps EGFP expression linked to the egr3 gene during zebrafish brain development, creating an atlas of telencephalic changes.

Keywords:
atlasegr3eversiontelencephalontelencephalon developmentzebrafish

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Area of Science:

  • Neuroscience
  • Developmental Biology
  • Genetics

Background:

  • The zebrafish telencephalon undergoes a two-step eversion process between 1 and 5 days post-fertilization (dpf).
  • The impact of telencephalic eversion on the precise positioning of neuronal populations and overall structural organization remains poorly understood.

Purpose of the Study:

  • To characterize the neurochemistry, cell cycle state, and morphology of an EGFP-positive cell population in the developing zebrafish telencephalon during eversion.
  • To map the transgene insertion site and validate EGFP expression against endogenous gene patterns.
  • To create a developmental atlas of the zebrafish telencephalon during eversion using advanced imaging and registration techniques.

Main Methods:

  • Utilized the Et(gata2:EGFP)bi105 transgenic zebrafish line to track EGFP expression.
  • Mapped the transgene insertion to the early-growth-response-gene-3 (egr3) locus.
  • Combined gata2:EGFP bi105 with other transgenes and structural markers for comprehensive cell tracking.
  • Registered developmental datasets to reference brains to create a telencephalic development atlas at 1, 2, and 5 dpf.
  • Compared expression patterns to adult stages and the Zebrafish Brain Browser reference brain.

Main Results:

  • Confirmed that EGFP expression in the Et(gata2:EGFP)bi105 line recapitulates endogenous egr3 expression in the pallial telencephalon.
  • Developed a detailed atlas documenting the morphological changes and cell population development during zebrafish telencephalic eversion.
  • Provided a framework for comparing developmental expression patterns with existing anatomical data.

Conclusions:

  • The gata2:EGFP bi105 transgene serves as a reliable marker for studying egr3 expression during zebrafish telencephalic development.
  • The generated atlas provides crucial insights into how telencephalic eversion influences cellular organization during early zebrafish brain development.
  • This work facilitates future research on zebrafish telencephalon structure and development by integrating molecular and anatomical data.