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The indirect method in the establishment of reference intervals for complement 3 and complement 4: A retrospective

Jiatong Chai1, Zeyu Sun1, Dongyang Xing1

  • 1Department of Laboratory Medicine, The First Hospital of Jilin University, Changchun, China.

Journal of Taibah University Medical Sciences
|June 20, 2022
PubMed
Summary

This study established new reference intervals (RIs) for complement 3 (C3) and complement 4 (C4) using an indirect method. The findings provide valuable RIs for C3 and C4, aiding clinical diagnosis in the local population.

Keywords:
ChinaData miningIndirect methodReference intervalsSerum complement 3Serum complement 4

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Area of Science:

  • Clinical Chemistry
  • Immunology
  • Biostatistics

Background:

  • Reference intervals (RIs) for complement 3 (C3) and complement 4 (C4) are infrequently established, particularly using indirect methodologies.
  • Accurate RIs are crucial for reliable clinical diagnosis and patient management.

Purpose of the Study:

  • To establish regional reference intervals (RIs) for complement 3 (C3) and complement 4 (C4) in the Chinese population.
  • To validate the indirect method for establishing C3 and C4 RIs.

Main Methods:

  • Utilized a large dataset (12,313 for C3, 12,125 for C4) from a Chinese hospital database.
  • Applied Tukey and Box-Cox standardization, LMS curves for age-related analysis, and a z-test.
  • Employed a non-parametric approach for RI determination.

Main Results:

  • Established reference intervals for C3 (0.83-1.58 g/L) and C4 (0.15-0.40 g/L).
  • Found no significant sex-based differences in C3 and C4 concentrations.
  • Observed a weak correlation between C3/C4 levels and age, with no significant age-related differences after statistical adjustment.

Conclusions:

  • Successfully established suitable and verified reference intervals for C3 and C4 for the local population.
  • Demonstrated the feasibility and practicality of the indirect method for establishing complement RIs.
  • The new RIs are expected to benefit clinical diagnosis and laboratory practice.