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A Nonsequencing Approach for the Rapid Detection of RNA Editing
Published on: April 21, 2022
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PRES: a webserver for decoding the functional perturbations of RNA editing sites
Dezhong Lv1, Kang Xu1, Changbo Yang1
1College of Bioinformatics Science and Technology, Harbin Medical University, Harbin, Heilongjiang 150081, China.
Briefings in Bioinformatics
|June 20, 2022
Summary
This study introduces the PRES webserver to analyze RNA editing sites, aiding in understanding their functional roles in diseases. It decodes functional perturbations from editome profiling for better disease mechanism insights.
Area of Science:
- Genomics
- Bioinformatics
- Molecular Biology
Background:
- Adenosine to inosine (A-to-I) RNA editing is quantifiable via RNA sequencing and computational tools.
- Altered RNA editing sites are prevalent in various human diseases, but their functional implications are not well understood.
Purpose of the Study:
- To develop a webserver, Perturbations of RNA Editing Sites (PRES), for decoding the functional consequences of altered RNA editing.
- To provide a platform for analyzing editome profiles and identifying disease-associated RNA editing events.
Main Methods:
- PRES utilizes editome profiling to annotate RNA editing sites and detect differential editing events.
- It characterizes functional perturbations including changes in miRNA/RNA binding protein regulation, RNA/protein structure, and biological pathways.
- A prioritization module ranks genes based on the functional impact of RNA editing.
Main Results:
- The PRES webserver offers user-friendly functionalities for analyzing RNA editing events.
- It provides efficient calculation, visualization of annotated editing events, filtering options, and application notebooks.
- The tool facilitates the characterization of functional perturbations linked to differential RNA editing.
Conclusions:
- PRES enables a comprehensive understanding of functional perturbations arising from altered RNA editing sites.
- This resource is expected to advance the study of RNA editing mechanisms in human complex diseases.
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