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Mesenchymal Stem Cells

Mesenchymal Stem Cells

Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...

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Isolation of Rat Adipose Tissue Mesenchymal Stem Cells for Differentiation into Insulin-producing Cells

Isolation of Rat Adipose Tissue Mesenchymal Stem Cells for Differentiation into Insulin-producing Cells

Published on: August 29, 2022

Fatty acids of type 2 diabetic serum decrease the stemness properties of human adipose-derived mesenchymal stem

Parisa Fayyazpour^1,2, Effat Alizadeh³, Vahid Hosseini⁴

¹Endocrine Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

Journal of Cellular Biochemistry

|June 20, 2022

Summary

Diabetic serum free fatty acids (FFAs) negatively impact adipose-derived mesenchymal stem cells (AdMSCs), reducing their stemness and regenerative potential. This finding highlights a potential barrier to using AdMSCs for type 2 diabetes treatment.

Keywords:

diabetes mellitus free fatty acids gas-liquid chromatography mesenchymal stem cells

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Creation and Transplantation of an Adipose-derived Stem Cell ASC Sheet in a Diabetic Wound-healing Model

Creation and Transplantation of an Adipose-derived Stem Cell ASC Sheet in a Diabetic Wound-healing Model

Published on: August 4, 2017

Area of Science:

Biomedical Science
Regenerative Medicine
Endocrinology

Background:

Type 2 diabetes is characterized by dyslipidemia and elevated serum free fatty acids (FFAs), which worsen insulin secretion and disease progression.
Adipose-derived mesenchymal stem cells (AdMSCs) are crucial in regenerative medicine and show promise for diabetes therapy.

Purpose of the Study:

To investigate the impact of diabetic serum FFAs on the stemness and regenerative properties of AdMSCs.
To compare the effects of FFAs from diabetic versus nondiabetic menopausal women's serum on AdMSCs.

Main Methods:

AdMSCs were exposed to FFAs isolated from diabetic and nondiabetic serum.
Gas-liquid chromatography analyzed serum FFA composition.
Real-time PCR assessed stemness markers (CD49e, CD90) and Wnt signaling genes (Axin-2, c-Myc).
BrdU assay, crystal violet staining, and fluorescence staining measured proliferation, colony formation, and glutathione (GSH) levels.

Main Results:

Diabetic serum exhibited a higher percentage of oleate compared to nondiabetic serum.
Diabetic FFAs significantly reduced CD90 and c-Myc expression, proliferation rates, colony formation capacity, and GSH levels in AdMSCs.
Specific reductions included: CD90 (-51%), c-Myc (-48%), proliferation (-35%), colony formation (-50%), and GSH (-62%).

Conclusions:

Diabetic serum FFAs impair the stemness characteristics of AdMSCs.
These negative effects may compromise the regenerative capabilities of AdMSCs, posing a challenge for their therapeutic application in type 2 diabetes.