Dissipation and risk assessment of fluopyram and trifloxystrobin on onion by GC-MS/MS

The dissipation and risk assessment studies on fluopyram, trifloxystrobin and their metabolites were carried out on onion under field conditions after two treatments of fluopyram 250 g/L + trifloxystrobin 250 g/L SC @ 150 and 300 g a.i. ha^-1. The onion bulb samples were collected at 0, 3, 7, 14, and 21 days after second spray to study the pattern of dissipation using QuEChERS methodology for processing and analysis on GC-MS/MS. The total initial residues of fluopyram (fluopyram + fluopyram benzamide) in immature onion bulb were 2.14 and 4.93 mg kg^-1, at single and double dose, respectively. The residues of 0.02 and 0.06 mg kg^-1 persisted in the mature onion bulb collected at the harvest (30 days after treatment). The total initial residues of trifloxystrobin (trifloxystrobin + CGA 321113) in immature onion bulb were 0.65 and 1.97 mg kg^-1, at single and double dose, respectively, which reached < LOQ and 0.06 mg kg^-1 at the respective doses at the harvest time. Dissipation of fluopyram followed second-order kinetics with DT₅₀ values of 1.83 and 1.74 days, whereas trifloxystrobin followed first-order kinetics with DT₅₀ values of 4.73 and 4.78 days, at single and double dose respectively. Risk assessment in terms of hazard quotient was done to estimate the risk that can occur due to application of this combination pesticide. It was observed that even the spray at the double recommended dose could not have dietary risks on the consumers.