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Progressive neurogenic muscle atrophy with fasciculation in mice treated with IDPN
Abstract:
Spontaneous discharges recorded on the EMG appeared in experimental mice given repeated doses of IDPN according to a time schedule. Spontaneous discharges showing a polyphasic pattern and duration of over 10 ms were considered to be fasciculations. One group of mice, treated with IDPN for 4 weeks became active with an increase in muscle volume 2 months after initiation of experiment; spontaneous discharges were recorded in this group at 3-4 weeks after first IDPN injection and disappeared at 4 months. Histologically, neurogenic muscle atrophy, in terms of group atrophy of muscles, was seen in muscles of mice exhibiting spontaneous discharge patterns. Peripheral nerves showed marked atrophy with degeneration. Dissociation of neurofilaments and other organelles was observed. Axonal swellings, filled with numerous maloriented neurofilaments, were confirmed in the lumbar cord. IDPN given repeatedly to mice at a certain dose causes degeneration of the peripheral nerves and results in neurogenic muscle atrophy.
Insights
Repeated exposure to IDPN in mice induced spontaneous muscle fasciculations and neurogenic muscle atrophy. Peripheral nerve degeneration, characterized by axonal swelling, was observed, leading to muscle atrophy.
Area of Science:
- Neuroscience
- Toxicology
- Pathology
Background:
- Repeated exposure to certain chemicals can induce neurological disorders.
- Understanding the mechanisms of neurotoxicity is crucial for preventing nerve damage.
Purpose of the Study:
- To investigate the effects of repeated IDPN administration on peripheral nerves and muscles in mice.
- To characterize the histological and electrophysiological changes associated with IDPN exposure.
Main Methods:
- Experimental mice received repeated doses of IDPN over a 4-week period.
- Electromyography (EMG) was used to record spontaneous muscle discharges (fasciculations).
- Histological examination of peripheral nerves and muscles was performed.
Main Results:
- Spontaneous, polyphasic fasciculations (>10 ms duration) were observed on EMG starting at 3-4 weeks post-injection.
- Mice exhibited increased activity and muscle volume 2 months after treatment initiation.
- Histology revealed neurogenic muscle atrophy, peripheral nerve degeneration, and axonal swellings with disoriented neurofilaments in the lumbar cord.
Conclusions:
- Repeated IDPN administration causes peripheral nerve degeneration.
- This degeneration leads to neurogenic muscle atrophy in experimental mice.
- IDPN-induced neurotoxicity affects both peripheral nerves and muscles.