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Updated: Sep 7, 2025

Evaluation of Antimicrobial Activities of Nanoparticles and Nanostructured Surfaces In Vitro
Published on: April 21, 2023
Antibacterial effect of singlet oxygen depending on bacteria surface charge
Il Kwon Bae1, Jun-Yong Shin2, Jae-Hak Son3
1Department of Companion Animals, Silla University, Busan 46958, South Korea.
Abstract:
Here, we investigated the bactericidal effects of two types of photoinduced reactive oxygen species (ROS), superoxide anion and singlet oxygen, on bacteria with distinct surface charges. We fabricated photofunctional polymer films (PFPFs) capable of generating both types of ROS, and they were subjected to photodynamic inactivation tests for 12 various strains of Acinetobacter baumannii. The results showed that the type I ROS (superoxide anion) was significantly dependent on the surface charge of the bacteria owing to charge-charge repulsion, while the type II ROS (singlet oxygen) was independent of the surface charge of the bacteria. These results could be significant in enhancing treatment efficiency in the clinical field.
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