Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Deep Learning Based on B-Mode and Color Doppler Ultrasound for Differentiation of Primary Thyroid Lymphoma and Hashimoto's Thyroiditis: A Retrospective Single-Center Study.

Diagnostics (Basel, Switzerland)·2026

Same author

Rectal endometriosis mimicking rectal cancer.

Gastroenterologia y hepatologia·2026

Same author

Kinetics of Transient Tissue Transparency via Refractive Index Engineering (T3RIE) for In Vivo Optical Imaging.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026

Same author

Commissioning and clinical implementation of Monaco treatment planning system on a compact pencil beam scanning proton therapy system.

Journal of applied clinical medical physics·2026

Same author

A Novel Bioluminescence Tomography System Compatible with CBCT-guided Small Animal Irradiators for High-Precision Preclinical Radiation Research.

Radiation research·2026

Same author

Treatment planning comparison of proton arc therapy and intensity modulated proton therapy for synchronous bilateral breast or chest wall and regional nodal irradiation.

Physics and imaging in radiation oncology·2026

Same journal

Method of spatial scanning of modulated laser radiation for outline imaging of interphalangeal joints.

Journal of biomedical optics·2026

Same journal

Multimodal optical imaging for the assessment of the teratogenic effects of ethanol on zebrafish development.

Journal of biomedical optics·2026

Same journal

Fluorescence properties of collagen types I-V: a comprehensive study of spectral and lifetime characteristics.

Journal of biomedical optics·2026

Same journal

Spectral dependence of lipofuscin fluorescence lifetimes revealed by FLIM with a superconducting nanowire single-photon detector.

Journal of biomedical optics·2026

Same journal

Building the future of biophotonics through experiential education and seasonal schools.

Journal of biomedical optics·2026

Same journal

Time-of-flight fluorescence depth mapping using a spatiotemporal deep learning model.

Journal of biomedical optics·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Sep 7, 2025

Bioluminescence-Based Tumor Quantification Method for Monitoring Tumor Progression and Treatment Effects in Mouse Lymphoma Models

Bioluminescence-Based Tumor Quantification Method for Monitoring Tumor Progression and Treatment Effects in Mouse Lymphoma Models

Published on: July 7, 2016

Quantitative molecular bioluminescence tomography.

Alexander Bentley^1,2, Xiangkun Xu^3,4, Zijian Deng^3,4

¹University of Birmingham, School of Computer Science, College of Engineering and Physical Sciences,, United Kingdom.

Journal of Biomedical Optics

|June 21, 2022

Summary

This summary is machine-generated.

This study introduces an optimization algorithm for bioluminescence imaging and tomography (BLT) that accurately determines bioluminescence source location and optical properties simultaneously. This method enhances quantitative molecular imaging accuracy in preclinical research.

Keywords:

bioluminescence imaging bioluminescence tomography diffuse optical imaging

More Related Videos

Monitoring Tumor Metastases and Osteolytic Lesions with Bioluminescence and Micro CT Imaging

Monitoring Tumor Metastases and Osteolytic Lesions with Bioluminescence and Micro CT Imaging

Published on: April 14, 2011

Bioluminescent Bacterial Imaging In Vivo

Bioluminescent Bacterial Imaging In Vivo

Published on: November 4, 2012

Related Experiment Videos

Last Updated: Sep 7, 2025

Bioluminescence-Based Tumor Quantification Method for Monitoring Tumor Progression and Treatment Effects in Mouse Lymphoma Models

Bioluminescence-Based Tumor Quantification Method for Monitoring Tumor Progression and Treatment Effects in Mouse Lymphoma Models

Published on: July 7, 2016

Monitoring Tumor Metastases and Osteolytic Lesions with Bioluminescence and Micro CT Imaging

Monitoring Tumor Metastases and Osteolytic Lesions with Bioluminescence and Micro CT Imaging

Published on: April 14, 2011

Bioluminescent Bacterial Imaging In Vivo

Bioluminescent Bacterial Imaging In Vivo

Published on: November 4, 2012

Area of Science:

Biomedical Imaging
Optical Physics
Preclinical Research

Background:

Bioluminescence imaging and tomography (BLT) are vital for studying biological activity in vivo, often using mouse models.
A key limitation of BLT is the unknown optical properties of the tissue, which necessitates estimations that reduce quantitative accuracy.

Purpose of the Study:

To develop an optimization algorithm for BLT that simultaneously recovers optical properties and bioluminescence source location.
To improve the quantitative accuracy of BLT without prior knowledge of optical parameters.

Main Methods:

A multi-parameter optimization algorithm was developed to process surface measurements.
The algorithm was validated using data from implanted light sources and an orthotopic glioblastoma mouse model.

Main Results:

The algorithm successfully recovered the size and location of bioluminescence sources, accounting for tissue attenuation.
Localization accuracy below 1 mm was achieved, comparable or superior to existing methods.

Conclusions:

Quantitative BLT is achievable using this approach without requiring prior optical parameter knowledge.
This method advances quantitative molecular imaging for assessing biological tumor functionality in vivo.