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Effect of CDCA5 on Proliferation and Metastasis of Triple Negative Breast Cancer Cells under shRNA Interference
Yilin Li1, Wei Peng2, Yunfang Deng3
1Department of Pathlology, Hunan Cancer Hospital, Changsha, 410013 Hunan Province, China.
Objective:
It was to explore the effect of cell division cycle associated 5 (CDCA5) under shRNA interference on proliferation and metastasis of triple negative breast cancer (TNBC) cells.
Methods:
MDA-ME-231 and BT549 cells were selected as the research objects. According to the different interference methods and CDCA5 interference sequences, they were divided into the interference group 1MDA-ME-231, the interference group 2MDA-ME-231, the interference group 1BT549, the interference group 2BT549 (using shRNA technology), the control group MDA-ME-231, and the control group BT549 (breast cancer cells under normal culture conditions). MCF10A cells were routinely cultured as the negative control group to analyze the effect of CDCA5 expression on the proliferation and migration of cancer cells.
Results:
The expression of CDCA5 protein in MDA-ME-231 and BT549 cells in control group was significantly higher than that in negative control group (P < 0.05). Compared with the control group, the inhibition rates of CDCA5 expression in 1MDA-ME-231, 2MDA-ME-231, 1BT549, and 2BT549 cells in the interference group were 39.01%, 42.98%, 49.57%, and 60.98%, respectively (P < 0.05). From 12 h, the proliferation level of TNBC cells at different culture time was lower than that of the control group (P < 0.05). Compared with the number of staining cells in the control group, the positive staining cells in 1MDA-ME-231 (61.42%), 2MDA-ME-231 (72.06%), 1BT549 (52.53%), and 2BT549 (59.65%) in the interference group were significantly decreased (P < 0.05).
Conclusion:
The results show that the expression of CDCA5 in TNBC is increased, which plays an important role in the proliferation and migration of cancer cells. shRNA interference technology can knock down the expression of CDCA5 and inhibit its "promoting cancer" effect.
Insights
Cell division cycle associated 5 (CDCA5) promotes triple-negative breast cancer (TNBC) proliferation and metastasis. shRNA interference effectively reduced CDCA5 expression, inhibiting TNBC cell growth and migration.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Triple-negative breast cancer (TNBC) is an aggressive subtype with limited targeted therapies.
- Cell division cycle associated 5 (CDCA5) is implicated in cell proliferation and cancer progression.
Purpose of the Study:
- To investigate the role of CDCA5 in TNBC proliferation and metastasis.
- To evaluate the efficacy of shRNA interference in targeting CDCA5 in TNBC cells.
Main Methods:
- Utilized shRNA technology to interfere with CDCA5 expression in MDA-MB-231 and BT549 TNBC cell lines.
- Cultured MCF10A cells as a negative control.
- Assessed cell proliferation and migration levels.
Main Results:
- CDCA5 protein expression was significantly higher in TNBC cells compared to normal cells.
- shRNA interference led to substantial inhibition of CDCA5 expression (39-61%).
- Reduced CDCA5 expression significantly decreased TNBC cell proliferation and migration.
Conclusions:
- CDCA5 plays a crucial role in promoting TNBC proliferation and metastasis.
- shRNA-mediated knockdown of CDCA5 effectively inhibits its oncogenic functions in TNBC.
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