Genome-Wide DNA Methylation Protocol for Epigenetics Studies

Area of Science:

• Epigenetics and Molecular Biology
• Genomics and Bioinformatics

Background:

• Epigenetic modifications are heritable changes in gene expression not encoded by DNA sequence.
• DNA methylation, forming 5-methylcytosine (5mC) via DNA methyltransferases, is a key epigenetic mechanism.
• DNA methylation influences chromatin structure and can down-regulate gene expression, impacting biological processes and diseases.

Purpose of the Study:

• To present a standardized protocol for DNA sample preparation.
• To enable genome-wide DNA methylation analysis using next-generation sequencing (NGS).
• To facilitate research into the role of DNA methylation in biological processes and diseases.

Main Methods:

• Development of a DNA sample preparation protocol.
• Adaptation of the protocol for compatibility with NGS workflows.
• Validation of the protocol for genome-wide methylation analysis.

Main Results:

• A robust method for obtaining DNA samples suitable for NGS sequencing was established.
• The protocol enables efficient and accurate genome-wide DNA methylation profiling.
• The method is ready for application in diverse research settings.

Conclusions:

• The presented protocol provides a crucial tool for advancing DNA methylation research.
• This method supports the investigation of epigenetic regulation in health and disease.
• Genome-wide methylation analysis via NGS is made more accessible.