Related Experiment Video

Updated: Sep 7, 2025

Comprehensive Analysis of Procoagulant Platelets Exhibiting Features of Necrosis, Apoptosis and Platelet Activation
04:37

Comprehensive Analysis of Procoagulant Platelets Exhibiting Features of Necrosis, Apoptosis and Platelet Activation

Published on: May 23, 2025

629

CPT2 K79 acetylation regulates platelet life span

Xuemei Fan1, Yang Wang1, Xiaohong Cai2

  • 1Department of Biochemistry and Molecular Cell Biology, and.

Blood Advances
|June 21, 2022
PubMed

Insights

Platelet storage lesion is caused by long-chain acylcarnitines (LCACs) accumulation, which damages mitochondria. Targeting LCAC generation improves platelet storage and extends posttransfusion survival.

Area of Science:

  • Biochemistry
  • Hematology
  • Cellular Biology

Background:

  • Platelet transfusion services face challenges due to the short lifespan of platelets.
  • Mitochondrial damage and platelet storage lesion contribute to reduced platelet viability.

Purpose of the Study:

  • To investigate the role of long-chain acylcarnitines (LCACs) in platelet storage lesion.
  • To identify mechanisms underlying LCAC accumulation and mitochondrial damage in platelets.
  • To explore therapeutic strategies for improving platelet storage quality.

Main Methods:

  • Investigated the link between fatty acid oxidation, AMP-activated protein kinase (AMPK)/acetyl-CoA carboxylase/carnitine palmitoyltransferase 1 (CPT1) pathways, and LCAC accumulation.
  • Elucidated the role of NAD+ exhaustion, sirtuin 3 (Sirt3) activity, and CPT2 acetylation in blocking fatty acid oxidation.
  • Tested inhibitors of AMPK or CPT1, Sirt3 agonists, and antioxidants in vitro and in vivo models.

Main Results:

  • Accumulation of LCACs was identified as a key factor in mitochondrial damage and platelet storage lesion.
  • Blockade of fatty acid oxidation, driven by AMPK/CPT1 pathway activation, leads to LCAC accumulation.
  • CPT2 K79 acetylation, resulting from NAD+ depletion and decreased Sirt3 activity, inhibits fatty acid oxidation and promotes LCAC buildup.
  • Interventions targeting LCAC generation significantly reduced platelet storage lesion and improved posttransfusion survival.

Conclusions:

  • CPT2 acetylation impairs fatty acid oxidation, worsening platelet storage lesion.
  • Targeting CPT2 acetylation presents a novel therapeutic strategy for enhancing platelet storage quality and extending platelet lifespan.

More Related Videos

Preparation of Washed Human Platelets for Quantitative Metabolic Flux Studies07:06

Preparation of Washed Human Platelets for Quantitative Metabolic Flux Studies

Published on: January 10, 2025

681
LC-MS Analysis of Human Platelets as a Platform for Studying Mitochondrial Metabolism06:04

LC-MS Analysis of Human Platelets as a Platform for Studying Mitochondrial Metabolism

Published on: April 4, 2016

11.3K

Related Concept Videos

Structure and Function of Platelets01:18

Structure and Function of Platelets

The cell fragments known as platelets are disc-shaped, with an average diameter of about 3 μm and a thickness of roughly 1 μm. They play a crucial role in the body's vascular clotting system, which also involves plasma proteins, blood cells, and blood vessel tissues.
Platelets are continually replenished, circulating in the bloodstream for 9-12 days before being removed by phagocytes, primarily in the spleen. A microliter of circulating blood contains between 150,000 and 450,000...
1.5K
Antiplatelet Drugs: Prostaglandin Synthesis, P2Y12 and Glycoprotein IIb/IIIa Inhibitors01:20

Antiplatelet Drugs: Prostaglandin Synthesis, P2Y12 and Glycoprotein IIb/IIIa Inhibitors

Antiplatelet drugs emerge as frontline defenders against the insidious threat of thromboembolic diseases, where abnormal clots obstruct vital blood vessels. These drugs stand as bulwarks, inhibiting platelet aggregation and clot formation, thereby mitigating the risk of life-threatening conditions like myocardial infarction, coronary artery disease, and thrombotic strokes.
Prostaglandin synthesis inhibitors, exemplified by the widely known aspirin, wield their power by irreversibly acetylating...
640
Formation of the Platelet Plug01:22

Formation of the Platelet Plug

The platelet phase, the second stage of hemostasis, commences around 15-20 seconds after an injury. It follows and overlaps with the vascular phase, during which blood vessels constrict to minimize blood loss.
As the injured blood vessel contracts, endothelial cells undergo contraction, revealing collagen fibers in the basement membrane and underlying connective tissue. Furthermore, the plasma membrane of endothelial cells becomes adhesive, preparing the site for platelet adhesion. Platelets...
7.0K
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
7.1K
Allosteric Proteins-ATCase01:19

Allosteric Proteins-ATCase

Binding sites linkages can regulate a protein's function.  For example, enzyme activity is often regulated through a feedback mechanism where the end product of the biochemical process serves as an inhibitor.
Aspartate transcarbamoylase (ATCase) is a cytosolic enzyme that catalyzes the condensation of L-aspartate and carbamoyl phosphate to  N-carbamoyl-L-aspartate. This reaction is the first step in pyrimidine biosynthesis. UTP and CTP, the end products of the pyrimidine synthesis...
5.9K