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Defined positive charge patterns created on DNA nanostructures determine cellular uptake efficiency.

Yiwei Shi1, Xuemei Xu2, Huaibin Yu1

  • 1Hubei Engineering Research Center for Biomaterials and Medical Protective Materials, Hubei Key Laboratory of Bioinorganic Chemistry and Materia Medica, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074, People's Republic of China.

Nano Letters
|June 21, 2022
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Summary
This summary is machine-generated.

Researchers developed a method to create patterned nanoparticles (NPs) and found edge-localized positive charges enhance cellular uptake. High-density charges also improved NP penetration into 3D cell structures.

Keywords:
DNA origamicharge patternsin situ polymerizationnano-bio interactions

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Area of Science:

  • Nanotechnology
  • Biomaterials Science
  • Surface Chemistry

Background:

  • Nanoparticle (NP) surface charge is critical for nano-bio interactions.
  • Controlling charge distribution on nanostructures remains a challenge.
  • Understanding charge effects on cellular uptake is essential for nanomedicine.

Purpose of the Study:

  • To develop a method for creating DNA nanostructures with defined charge patterns below 100 nm.
  • To investigate how charge density and location influence nanoparticle cellular uptake.
  • To explore the impact of charge patterns on NP penetration into 3D multicellular spheroids.

Main Methods:

  • Fabrication of negatively charged polymer nanopatterns on DNA origami via in situ polymerization.
  • Generation of positive charges on immobilized polymers using photoresponsive monomers and irradiation.
  • Characterization of charge patterns and assessment of cellular uptake efficiency and spheroid penetration.

Main Results:

  • Successful creation of DNA nanostructures (<100 nm) with precise, charge-separated patterns.
  • Positively charged patterns on nanostructure edges significantly enhanced cellular uptake compared to central patterns.
  • High-density positive charge decoration improved nanoparticle penetration into 3D multicellular spheroids.

Conclusions:

  • The developed method allows for the construction of elaborate charge-separated substructures on NP surfaces.
  • Nanostructure edge charge localization is a key factor for efficient cellular uptake.
  • Surface charge distribution significantly impacts nano-bio interactions, offering new strategies for nanomedicine development.