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Low-energy green light alleviates senescence-like phenotypes in a cell model of photoaging
Chuanlong Jia1, Chengchen Gong1, Yongzhou Lu1
1Department of Dermatology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China.
Background:
Ultraviolet B (UVB) affects diverse pathways in skin cells, resulting in skin photoaging. Skin fibroblasts internalize and degrade elastin and collagen, playing prominent roles in photoaging. Green light is used in many fields of dermatology, but few studies have examined its role in photoaging. The present work aimed to assess low-energy green light for its effects in a previously proposed cell model of photoaging and to explore the possible anti-photoaging mechanism.
Methods:
The stress-induced premature senescence (SIPS) model was constructed via repeated treatment of MDFs with UVB. Senescence-like phenotypes were compared among normal, low-energy green light pretreatment and UVB groups, for example, cell morphological properties, senescence-associated β-galactosidase (SA-β-gal) amounts, extracellular matrix (ECM) biosynthesis and degradation, and autophagy.
Results:
In comparison with the UVB group, the green light pretreatment group showed significantly decreased number of senescent mast cells and markedly declined signal intensity and amounts of SA-β-gal-positive cells. Furthermore, green light pretreatment directly affected ECM by increasing type I and type III collagen production and decreasing MMP-1 amounts. Moreover, changes in autophagy levels induced by green light pretreatment provided a potential mechanism underlying its anti-aging property.
Conclusions:
Low-energy green light pretreatment improves senescence-like phenotypes in vitro, indicating a possible application for anti-aging in clinic after future research has uncovered the potential mechanism.
Insights
Low-energy green light pretreatment reduces skin cell senescence and improves markers of photoaging. This suggests a potential clinical application for green light therapy in anti-aging treatments.
Area of Science:
- Dermatology
- Cell Biology
- Photobiology
Background:
- Ultraviolet B (UVB) radiation induces skin photoaging by affecting skin cell pathways, notably collagen and elastin degradation in fibroblasts.
- While green light has dermatological applications, its specific role in mitigating photoaging remains underexplored.
- This study investigates green light's potential anti-aging effects on a cellular model of photoaging.
Purpose of the Study:
- To evaluate the efficacy of low-energy green light pretreatment in a cellular model of skin photoaging.
- To elucidate the underlying mechanisms of green light's anti-photoaging effects, including its impact on extracellular matrix and autophagy.
Main Methods:
- A stress-induced premature senescence (SIPS) model was established using repeated UVB exposure on human dermal fibroblasts (MDFs).
- Senescence-like phenotypes were assessed, comparing normal cells, green light-pretreated cells, and UVB-exposed cells.
- Key markers analyzed included cell morphology, senescence-associated β-galactosidase (SA-β-gal) activity, extracellular matrix (ECM) synthesis and degradation, and autophagy levels.
Main Results:
- Green light pretreatment significantly reduced the number of senescent cells and SA-β-gal-positive cells compared to UVB exposure alone.
- Green light enhanced the production of type I and type III collagen while decreasing matrix metalloproteinase-1 (MMP-1) levels, indicating improved ECM integrity.
- Alterations in autophagy pathways were observed, suggesting a potential molecular mechanism for the observed anti-aging effects.
Conclusions:
- Low-energy green light pretreatment effectively ameliorates senescence-like phenotypes in skin cells in vitro.
- These findings support the potential of green light as a novel therapeutic approach for clinical anti-aging applications.
- Further research is warranted to fully understand and optimize the anti-photoaging mechanisms of green light therapy.
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