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RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
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Related Experiment Video

Updated: Sep 7, 2025

Double-stranded RNA Oral Delivery Methods to Induce RNA Interference in Phloem and Plant-sap-feeding Hemipteran Insects
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Agrochemical control of gene expression using evolved split RNA polymerase.

Yuan Yuan1, Jin Miao2

  • 1Department of Neurophysiology and Neuropharmacology, Institute of Special Environmental Medicine and Co-innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu Province, China.

Peerj
|June 22, 2022
PubMed
Summary

Researchers developed a novel mandipropamid-induced gene expression system (Mandi-T7) for precise control. This system enables gene expression in bacteria within plant tissues, overcoming limitations of existing methods.

Keywords:
Inducible gene expressionMandipropamidT7 RNAP

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Area of Science:

  • Synthetic Biology
  • Molecular Biology
  • Biotechnology

Background:

  • Chemically-inducible gene expression systems are crucial for controlling gene activity in research and industry.
  • Current systems often lack specificity, being limited to particular organisms, hindering inter-organismal studies.
  • Controlling gene expression across different species, especially within complex environments like plant tissues, remains a challenge.

Purpose of the Study:

  • To engineer a novel chemically-inducible gene expression system with broader applicability.
  • To demonstrate the utility of this new system for controlling gene expression in bacteria residing within plant tissues.
  • To overcome the limitations of existing inducible systems in studying inter-organismal interactions.

Main Methods:

  • Engineered a mandipropamid-inducible system (Mandi-T7) utilizing an evolved split T7 RNA polymerase (RNAP).
  • Tested the system's efficacy by inducing Green Fluorescent Protein (GFP) expression.
  • Performed proof-of-principle experiments with *E. coli* cells cultured within plant tissue.

Main Results:

  • Successfully established a functional mandipropamid-inducible gene expression system (Mandi-T7).
  • Demonstrated successful induction of GFP expression in *E. coli*.
  • Achieved controlled gene expression in bacteria located inside plant tissue, validating the system's applicability in a complex biological context.

Conclusions:

  • The Mandi-T7 system provides a new tool for precise, chemical control of gene expression.
  • This system expands the possibilities for studying gene function and interactions in bacteria within plant environments.
  • The engineered system offers a valuable platform for both basic research and biotechnological applications requiring cross-organismal control.