Related Experiment Video

Updated: Jul 26, 2026

Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)
08:21

Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)

Published on: March 16, 2012

15.7K

Outbreak of IncX8 Plasmid-Mediated KPC-3-Producing Enterobacterales Infection, China

Lan Chen, Wenxiu Ai, Ying Zhou

    Emerging Infectious Diseases
    |June 22, 2022
    PubMed

    Insights

    An outbreak of rare KPC-3 carbapenem-resistant Enterobacterales (CRE) occurred in China, driven by IncX8 plasmids. These strains showed high virulence and rapid resistance development, necessitating global monitoring.

    Area of Science:

    • Microbiology
    • Infectious Diseases
    • Genomics

    Background:

    • Carbapenem-resistant Enterobacterales (CRE) are a significant global health threat.
    • Klebsiella pneumoniae carbapenemase (KPC)-2-producing CRE is common in China, but KPC-3 is rare.
    • An unusual outbreak of KPC-3-producing CRE was identified in China.

    Purpose of the Study:

    • To investigate an outbreak of KPC-3-producing Enterobacterales in China.
    • To characterize the genetic elements and transmission dynamics of blaKPC-3.
    • To assess the virulence and resistance potential of these emerging strains.

    Main Methods:

    • Detection and WGS analysis of 25 blaKPC-3-positive Enterobacteriale isolates.
    • Plasmid analysis, conjugation experiments, and virulence assessment using a Galleria mellonella model.
    • In vitro selection assay for ceftazidime/avibactam resistance.

    Main Results:

    • The outbreak involved 24 patients and 25 isolates between August 2020 and June 2021.
    • blaKPC-3 genes were located on IncX8 plasmids, facilitating interspecies transmission.
    • KPC-3-producing Serratia marcescens clonal expansion and plasmid transmission occurred.
    • K. pneumoniae strains with blaKPC-3 plasmids exhibited high virulence.
    • KPC-3 strains rapidly developed resistance to ceftazidime/avibactam in vitro.

    Conclusions:

    • This study reports a rare outbreak of KPC-3-producing CRE in China.
    • IncX8 plasmids play a crucial role in the spread of blaKPC-3 across different Enterobacterales species.
    • The high virulence and rapid resistance development underscore the need for close monitoring of these strains and plasmids globally.
    Keywords:
    ChinaEnterobacteralesKPC-3Klebsiella pneumoniaeantimicrobial resistancebacteriabacterial infectionscarbapenemasehorizontal transferoutbreakplasmidtransmission

    More Related Videos

    Generation of Marked and Markerless Mutants in Model Cyanobacterial Species11:45

    Generation of Marked and Markerless Mutants in Model Cyanobacterial Species

    Published on: May 29, 2016

    12.2K
    Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing09:44

    Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing

    Published on: December 23, 2022

    2.3K

    Related Concept Videos

    Investigation of Disease Outbreaks01:23

    Investigation of Disease Outbreaks

    Multistate foodborne outbreaks pose significant public health risks and require meticulous investigation to identify sources and implement control measures. The Centers for Disease Control and Prevention (CDC) utilizes a dynamic seven-step process for these investigations, integrating data from laboratories, interviews, and environmental assessments to protect public health.Outbreak Detection: The detection of multistate outbreaks typically begins with PulseNet, the CDC's national laboratory...
    Plague01:24

    Plague

    Plague is a highly virulent zoonotic disease caused by Yersinia pestis, a Gram-negative, facultatively anaerobic coccobacillus. This pathogen primarily circulates among rodent populations and is transmitted to humans through the bite of infected fleas. Additional transmission routes include direct contact with infected animal tissue or inhalation of respiratory droplets from individuals with pneumonic plague. These multiple transmission pathways highlight the bacterium’s potential for rapid...
    Bacterial Gastroenteritis01:18

    Bacterial Gastroenteritis

    Bacterial gastroenteritis, characterized by diarrhea, abdominal cramps, and vomiting, is often caused by ingestion of contaminated food or water and is frequently associated with pathogenic Escherichia coli strains. These microbes exploit two principal mechanisms to inflict disease.Shiga toxin–producing E. coli, also referred to as STEC—notably O157:H7—release Shiga toxins that target ribosomes, blocking protein synthesis. The B subunit of the toxin binds the host glycolipid receptor...